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首页> 外文期刊>Molecular and Cellular Biology >Role of SGP2, a suppressor of a gpa1 mutation, in the mating-factor signaling pathway of Saccharomyces cerevisiae.
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Role of SGP2, a suppressor of a gpa1 mutation, in the mating-factor signaling pathway of Saccharomyces cerevisiae.

机译：SGP2，gpa1突变的抑制剂，在酿酒酵母的交配因子信号通路中的作用。

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Loss of function of GPA1, which encodes a guanine-nucleotide-binding protein, arrests the cell at the G1 phase and allows it to mate, suggesting that the gpa1 mutation spontaneously exerts an intracellular signal that mimics the action of mating factor. We have cloned the SGP2 gene, which was first identified as a secondary mutation that allowed a gpa1::HIS3 mutant to grow and to show a non-cell-type-specific sterile phenotype. Disruption of SGP2 confers temperature-sensitive growth and a-specific sterile phenotypes, characteristics similar to those conferred by the dpr1 (ram) mutation, a suppressor of RAS2Val-19. The following observations indicate that SGP2 and DPR1 are in fact identical. (i) The cloned SGP2 complements both the temperature-sensitive growth and the a-specific sterility of the dpr1 mutant and can be integrated into the chromosomal DPR1 locus. (ii) The cloned DPR1, in turn, complements the ability of sgp2 to suppress the lethality of gpa1::HIS3. (iii) The dpr1 mutation suppresses the growth defect of gpa1::HIS3, and the dpr1 gpa1::HIS3 strain shows a non-cell-type-specific sterile phenotype. (iv) sgp2 is closely linked to the dpr1 locus. The DPR1 product has been shown to be responsible for processing and fatty acid acylation of a-factor and RAS proteins at their carboxyl termini. Therefore, the SGP2 (DPR1) product may be involved in membrane localization of an essential component in the mating-factor signaling pathway.
机译：GPA1编码鸟嘌呤核苷酸结合蛋白的功能丧失，使细胞停滞在G1期并使其交配，这表明gpa1突变自发地发出了模仿交配因子作用的细胞内信号。我们已经克隆了SGP2基因，该基因首先被鉴定为允许gpa1 :: HIS3突变体生长并显示出非细胞类型特异性无菌表型的二级突变。 SGP2的破坏赋予了温度敏感的生长和a特异的无菌表型，这些特征与dpr1（ram）突变（一种RAS2Val-19的抑制剂）所赋予的特征相似。以下观察结果表明SGP2和DPR1实际上是相同的。 （i）克隆的SGP2补充了dpr1突变体对温度敏感的生长和a特异不育性，并且可以整合到染色体DPR1基因座中。 （ii）克隆的DPR1反过来补充了sgp2抑制gpa1 :: HIS3杀伤力的能力。 （iii）dpr1突变抑制了gpa1 :: HIS3的生长缺陷，并且dpr1 gpa1 :: HIS3菌株显示出非细胞类型特异性的无菌表型。 （iv）sgp2与dpr1基因座紧密相连。已显示DPR1产物负责a因子和RAS蛋白在其羧基末端的加工和脂肪酸酰化。因此，SGP2（DPR1）产品可能参与交配因子信号通路中重要成分的膜定位。

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