U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals.

P Brennwald; G Porter; J A Wise

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U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals.

机译：U2小核RNA在粟酒裂殖酵母和哺乳动物之间非常保守。

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We report the molecular cloning and sequencing of the most abundant trimethylguanosine-capped small nuclear RNA from the fission yeast Schizosaccharomyces pombe, a highly conserved homolog of mammalian U2 small nuclear RNA. This RNA is 186 nucleotides in length, just 2 nucleotides shorter than its human counterpart; this is in contrast to Saccharomyces cerevisiae U2, which is 1,175 nucleotides long. Moreover, the secondary structure of Schizosaccharomyces pombe U2 is virtually identical to that of mammalian U2, including the 3' half of the RNA, which shows limited primary sequence identity. Northern (RNA) blot analysis revealed that the size of this RNA is conserved not only in fission yeasts but in many organisms, including other ascomycetes.

机译：我们从裂变酵母裂殖酵母粟酒裂殖酵母，最保守的哺乳动物U2小核RNA的同源物，报告了最丰富的三甲基鸟苷加帽的小核RNA的分子克隆和测序。这种RNA长度为186个核苷酸，仅比其人类对应物短2个核苷酸。这与酿酒酵母U2（长度为1,175个核苷酸）相反。此外，粟酒裂殖酵母U2的二级结构实际上与哺乳动物U2的二级结构相同，包括RNA的3'半部分，显示出有限的一级序列同一性。 Northern（RNA）印迹分析表明，不仅在裂变酵母中而且在许多生物体中，包括其他子囊菌中，该RNA的大小均得到保守。

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《Molecular and Cellular Biology》 |1988年第12期|共6页
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P Brennwald; G Porter; J A Wise;
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1. U1 small nuclear RNA from Schizosaccharomyces pombe has unique and conserved features and is encoded by an essential single-copy gene. [J] . G Porter, P Brennwald, J A Wise Molecular and Cellular Biology . 1990,第6期

机译：来自粟酒裂殖酵母的U1小核RNA具有独特且保守的特征，并由必需的单拷贝基因编码。
2. Pac1p, an RNase III homolog, is required for formation of the 3' end of U2 snRNA in Schizosaccharomyces pombe. [J] . Zhou D, Frendewey D, Lobo Ruppert-SM RNA . 1999,第8期

机译：在粟酒裂殖酵母中形成U2 snRNA的3'端需要RNase III同源物Pac1p。
3. Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae. [J] . S E Wells, M Ares Molecular and Cellular Biology . 1994,第9期

机译：需要高度保守的U2小核RNA结构与Prp5p，Prp9p，Prp11p和Prp21p蛋白之间的相互作用，以确保酿酒酵母中U2小核糖核蛋白的完整性。
4. Comparative analysis of RNA polymerase II reveals potential evolutionary conserved posttranslational modified sites amongst Schizosacharomyces pombe and Saccharomyces cerevisiae [C] . Selene Swanson, Charles Banks, Zhihui Wen, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：RNA聚合酶II的比较分析揭示了Schizosacharomyces Pombe和Saccharomyces Cerevisiae中的潜在进化潜在的后期改性位点
5. Carbon-13 nuclear magnetic resonance studies of D-glucose metabolism and purification of gluconokinase from Schizosaccharomyces pombe. [D] . Ye, Honggang. 1993

机译：碳13核磁共振研究D-葡萄糖代谢和粟酒裂殖酵母糖苷激酶的纯化。
6. U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals. [O] . P Brennwald, G Porter, J A Wise 1988

机译：U2小核RNA在粟酒裂殖酵母和哺乳动物之间非常保守。
7. U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals. [O] . Brennwald, P, Porter, G, Wise, J A 1988

机译：U2小核RNA在粟酒裂殖酵母和哺乳动物之间非常保守。

U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals.

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