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首页> 外文期刊>Molecular and Cellular Biology >Transcription of HLA class II genes in the absence of B-cell-specific octamer-binding factor.
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Transcription of HLA class II genes in the absence of B-cell-specific octamer-binding factor.

机译：在没有B细胞特异性八聚体结合因子的情况下转录HLA II类基因。

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HLA-DR and other human class II histocompatibility genes are expressed by Epstein-Barr virus-transformed B-lymphocyte cell lines but not by most T-cell leukemia lines. We determined by transcriptional run-on experiments that regulation of class II expression in these cells is at the level of gene transcription; nuclei isolated from B-cell lines actively transcribe class II mRNA, whereas nuclei from non-class II-expressing T-cell lines and from the class II transactive factor-deficient B-cell mutant 6.1.6 do not. In searching for DNA-binding proteins which might regulate transcription, we found both a ubiquitous (B1) and a B-cell-specific (B2) factor which bind to the octamer sequence ATTTGCAT 52 base pairs 5' of the cap site in the DR alpha gene. We examined the relationship of these factors to DR alpha transcription. HUT-78, a T-cell line which expresses class II mRNA constitutively, contains only the ubiquitous B1 octamer-binding factor also found in non-class II-expressing T-cell leukemias. Human fibroblast, HeLa, and melanoma cell lines similarly contain only the ubiquitous factor, even when these cells are induced to express class II mRNA by treatment with gamma interferon. Both B1 and B2 binding factors are present in the B-cell mutant 6.1.6, which nevertheless fails to transcribe class II mRNA. Although we have not ruled out the requirement of B-cell-specific octamer-binding factor B2 for class II expression in B cells, it is clear that in other cells substantial DR alpha transcription occurs in the absence of this factor.
机译：HLA-DR和其他人类II类组织相容性基因由爱泼斯坦-巴尔病毒转化的B淋巴细胞细胞系表达，但大多数T细胞白血病细胞系不表达。我们通过转录连续实验确定，这些细胞中II类表达的调节处于基因转录水平。从B细胞系中分离出的细胞核会主动转录II类mRNA，而从非表达II类的T细胞系和来自缺乏II类交易活性因子的B细胞突变体6.1.6的细胞核则不会转录。在寻找可能调节转录的DNA结合蛋白时，我们发现了普遍存​​在的（B1）和B细胞特异性（B2）因子都与DR中帽位的ATTAMCAT 52个碱基对的5'碱基对结合alpha基因。我们检查了这些因素与DRα转录的关系。 HUT-78是一种组成型表达II类mRNA的T细胞系，仅包含普遍存在的B1八聚体结合因子，该因子也在非II类表达T细胞白血病中发现。人类成纤维细胞，HeLa和黑色素瘤细胞系类似地仅包含遍在因子，即使通过γ干扰素处理诱导这些细胞表达II类mRNA。 B1和B2结合因子均存在于B细胞突变体6.1.6中，但仍无法转录II类mRNA。尽管我们没有排除B细胞特异性八聚体结合因子B2对于B细胞中II类表达的需求，但很明显，在其他细胞中，在没有该因子的情况下会发生大量DRα转录。

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