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首页> 外文期刊>Molecular and Cellular Biology >The Mu1 maize transposable element induces tissue-specific aberrant splicing and polyadenylation in two Adh1 mutants.
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The Mu1 maize transposable element induces tissue-specific aberrant splicing and polyadenylation in two Adh1 mutants.

机译：Mu1玉米转座因子在两个Adh1突变体中诱导组织特异性异常剪接和聚腺苷酸化。

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摘要

Insertions of the maize transposable element Robertson's Mutator (Mu) into intron 1 of the Adh1 gene have produced a number of mutant alleles altered in quantitative expression. It has previously been shown that transcription and mRNA accumulation are reduced for two of these alleles, Adh1-S3034 and Adh1-S4477. In this report, we describe the presence of Mu1-hybridizing polyadenylated transcripts in roots of anaerobically induced seedlings of these same mutants. Sequence analysis of Mu1-hybridizing clones from a cDNA library of S3034 RNA indicated that these transcripts originated from the Adh1 locus and were produced by alternative processing of S3034 pre-mRNA. Approximately half of the cDNAs represented transcripts that had not undergone excision of the intron containing the 1.4-kilobase Mu1 insertion but were processed in response to signals present in the transposable element. Mu1 contains a donor splice site in the 5'-terminal inverted repeat that can be joined to the Adh1 exon 2 acceptor, resulting in removal of most of the Mu1 sequences from the pre-mRNA; alternatively this donor can be spliced to an acceptor within Mu1, removing an 89-nucleotide intron. Mu1 also contains polyadenylation signals that are used to produce truncated transcripts. These Mu1 transcripts produced by aberrant splicing and polyadenylation were not detected in RNA isolated from developing kernels.
机译：玉米转座子罗伯逊突变体（Mu）插入Adh1基因的内含子1中已产生了数量突变的定量等位基因。先前已经显示，这些等位基因中的两个，Adh1-S3034和Adh1-S4477，转录和mRNA积累减少。在此报告中，我们描述了在厌氧诱导这些相同突变体的幼苗的根中存在Mu1杂交的聚腺苷酸转录物。来自S3034 RNA cDNA文库的Mu1杂交克隆的序列分析表明，这些转录本源自Adh1基因座，并通过S3034 pre-mRNA的替代加工产生。大约一半的cDNA代表未经过内含1.4碱基碱基Mu1插入的内含子切除，但响应转座因子中存在的信号而被处理的转录本。 Mu1在5'末端反向重复序列中包含一个供体剪接位点，该剪接位点可与Adh1外显子2受体连接，从而导致大多数mu1序列从前mRNA中去除。或者，可以将该供体剪接到Mu1内的受体上，从而除去89个核苷酸的内含子。 Mu1还包含用于产生截短转录本的聚腺苷酸化信号。这些异常的剪接和聚腺苷酸产生的Mu1转录物未在从发育中的籽粒中分离的RNA中检测到。

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