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首页> 外文期刊>Molecular and Cellular Biology >Organization and expression of the chicken N-myc gene.
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Organization and expression of the chicken N-myc gene.

机译：鸡N-myc基因的组织和表达。

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We cloned the chicken N-myc gene and analyzed its structure and expression. We found that it consisted of three exons with coding regions in exons 2 and 3. Comparison to mammalian N-myc genomic sequence indicated that nucleotide sequences of the 5'-flanking region, noncoding exon 1, and introns were not conserved, but coding and 3' noncoding sequences showed significant homology to mammalian N-myc. Alignment of deduced amino acid sequences of chicken and mammalian N-myc proteins revealed nine conserved domains interrupted by different lengths of nonhomologous sequences. Two of the domains were specific to N-myc proteins, and the other seven were common to c-myc proteins. Northern blot (immunoblot) and in situ hybridization analyses of 3.5-day-old chicken embryos revealed that high-level expression of the N-myc gene was confirmed to certain tissues, e.g., the central nervous system, neural crest derivatives, and mesenchyme of limb buds. In the beak and limb primordia, N-myc expression in the mesenchyme was higher toward the distal end, suggesting possible involvement in positional assignment of the tissue within the rudimentary structures.
机译：我们克隆了鸡N-myc基因，并分析了其结构和表达。我们发现它由三个外显子组成，在外显子2和3中具有编码区。与哺乳动物N-myc基因组序列的比较表明，5'侧翼区，非编码外显子1和内含子的核苷酸序列不保守，但编码和3'非编码序列显示出与哺乳动物N-myc的显着同源性。鸡肉和哺乳动物N-myc蛋白的推导氨基酸序列的比对揭示了9个保守结构域，被不同长度的非同源序列打断。其中两个结构域是N-myc蛋白特有的，其他七个结构域是c-myc蛋白所共有的。对3.5天大的鸡胚进行Northern blot（免疫印迹）和原位杂交分析表明，N-myc基因的高表达已在某些组织中得到证实，例如中枢神经系统，神经rest衍生物和间充质。肢芽。在喙和肢体原基中，间充质中的N-myc表达朝着远端较高，这表明可能参与了基本结构内组织的位置分配。

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