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首页> 外文期刊>Molecular and Cellular Biology >Most of the homeobox-containing Xhox 36 transcripts in early Xenopus embryos cannot encode a homeodomain protein.
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Most of the homeobox-containing Xhox 36 transcripts in early Xenopus embryos cannot encode a homeodomain protein.

机译：在非洲爪蟾早期胚胎中，大多数含有同源盒的Xhox 36转录物不能编码同源域蛋白。

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摘要

Multiple Xhox 36 transcripts accumulate in Xenopus embryos from gastrula to early tadpole stages. The transcripts were characterized by sequencing cDNA clones and by S1 protection and Northern (RNA) blotting of embryonic RNA with probes derived from the cDNAs. The Xhox 36 RNAs included unspliced precursor transcripts that accumulated in the embryonic nuclei, spliced transcripts that contained multiple stop codons in frame with the homeobox, and less abundant coding mRNAs. These transcripts were generated either by alternative splicing or multiple initiations from a single Xhox 36 gene. The sequence of a cDNA clone of the unspliced transcript showed that the intron contained a noncanonical 3' splice site. However, the intron was spliced efficiently when expressed from a plasmid injected into Xenopus embryos, suggesting that the inefficient splicing of the endogenous RNA is not due to the unusual 3' splice site. The accumulation of noncoding and unspliced transcripts suggests multiple levels of regulation in the embryonic expression of the Xhox 36 gene.
机译：从腹足到t早期的非洲爪蟾胚胎中积累了多个Xhox 36转录本。通过对cDNA克隆进行测序，并通过S1保护和用源自cDNA的探针对胚胎RNA进行Northern（RNA）印迹来表征转录物。 Xhox 36 RNA包括在胚核中积累的未剪接的前体转录物，与同源异型框一致的包含多个终止密码子的剪接的转录物，以及编码较少的mRNA。这些转录本是通过替代剪接或单个Xhox 36基因的多次启动产生的。未剪接的转录本的cDNA克隆的序列表明内含子含有一个非规范的3′剪接位点。但是，内含子从注射到非洲爪蟾胚胎中的质粒表达时能被有效剪接，这表明内源性RNA的剪接效率低下不是由于异常的3'剪接位点引起的。非编码和未剪接的转录物的积累表明，Xhox 36基因的胚胎表达中存在多种调控水平。

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