• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >M-CAT binding factor, a novel trans-acting factor governing muscle-specific transcription.
【24h】

M-CAT binding factor, a novel trans-acting factor governing muscle-specific transcription.

机译：M-CAT结合因子，一种控制肌肉特异性转录的新型反式作用因子。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The cardiac troponin T (cTNT) promoter contains a highly muscle specific distal promoter element capable of conferring muscle-specific transcription from a heterologous TATA box-transcription initiation site. Three sequence motifs within this distal promoter element are conserved in the promoter and regulatory regions of many sarcomeric protein genes. Mutational analysis demonstrated that homologies to two of these conserved motifs (CArG/CBAR and MEF 1) were not required for activity of cTNT promoter-marker gene constructs in transfected embryonic skeletal muscle cells. In contrast, disruption of either or both copies of the conserved M-CAT motif (5'-CATTCCT-3') inactivated the cTNT promoter in these cells. Both M-CAT motifs were protected from DNase I cleavage in solution footprint assays by an M-CAT binding factor (MCBF) present in nuclear extracts from embryonic muscle tissue. M-CAT mutations that inactivated the cTNT promoter also disrupted MCBF binding, indicating that MCBF may be a key trans-acting factor required for muscle-specific expression of the cTNT promoter. MCBF also bound to the M-CAT motif in the distal promoter region of the skeletal alpha-actin gene, suggesting that it may play a role in the regulation of this and perhaps other muscle genes that contain M-CAT motifs.
机译：心肌肌钙蛋白T（cTNT）启动子包含高度肌肉特异性的远端启动子元件，能够赋予来自异源TATA盒转录起始位点的肌肉特异性转录。该远端启动子元件内的三个序列基序在许多肌节蛋白基因的启动子和调节区中保守。突变分析表明，在转染的胚胎骨骼肌细胞中，cTNT启动子-标记基因构建体的活性不需要与这些保守基序中的两个（CArG / CBAR和MEF 1）同源。相反，保守的M-CAT基序（5'-CATTCCT-3'）的一个或两个拷贝的破坏使这些细胞中的cTNT启动子失活。两种M-CAT基序在溶液足迹测定中均受到保护，以防止DNA酶I裂解，这是通过存在于胚胎肌肉组织核提取物中的M-CAT结合因子（MCBF）来实现的。使cTNT启动子失活的M-CAT突变也破坏了MCBF结合，表明MCBF可能是cTNT启动子肌肉特异性表达所需的关键反式作用因子。 MCBF还与骨骼肌α-肌动蛋白基因的远端启动子区域中的M-CAT基序结合，表明它可能在调控该基因以及可能包含M-CAT基序的其他肌肉基因中发挥作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号