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An alternative non-tyrosine protein kinase product of the c-src gene in chicken skeletal muscle.

机译：鸡骨骼肌中c-src基因的另一种非酪氨酸蛋白激酶产物。

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While the c-src locus is expressed as a 4.0-kilobase (kb) mRNA coding for pp60c-src in various chicken tissues, including embryonic muscle, it is expressed as a novel 3.0-kb mRNA in adult skeletal muscle. We have analyzed the primary structure of this alternatively transcribed and spliced c-src mRNA. The sequence revealed three open reading frames, with the previously defined c-src exons 1 through 5 or 6 comprising the third, on the 3' untranslated region of this 3-kb mRNA. The exons coding for the tyrosine kinase domain of pp60c-src were excluded. On the 5' side, 2 kb of sequence upstream from the previously defined exon 1 of the c-src gene was included in this mRNA. The start site for the 3-kb mRNA probably lies downstream of that for the 4-kb mRNA. The first reading frame of the 3.0-kb mRNA, called sur (for src upstream region), encoded a 24-kilodalton (kDa) protein product rich in cysteine and proline residues. In vitro analysis indicated that the 24-kDa sur protein was membrane associated. Antibodies to sur protein detected in vivo a 24-kDa muscle-specific protein which was developmentally regulated and corresponded to the switch from the 4-kb to the 3-kb c-src mRNA. A striking kinetic pattern of appearance of sur protein and disappearance of pp60c-src suggests that the expression of these two proteins is inversely related.
机译：虽然c-src基因座在包括鸡胚肌肉在内的各种鸡组织中表达为pp60c-src的4.0 kb碱基对（kb）mRNA，但在成人骨骼肌中却表达为新的3.0 kb mRNA。我们已经分析了这种交替转录和剪接的c-src mRNA的主要结构。该序列在该3-kb mRNA的3'非翻译区上揭示了三个开放阅读框，其中先前定义的c-src外显子1至5或6包含第三个外显子。排除了编码pp60c-src酪氨酸激酶结构域的外显子。在5'端，此mRNA中包含c-src基因先前定义的外显子1上游2 kb的序列。 3-kb mRNA的起始位点可能位于4-kb mRNA的下游。 3.0-kb mRNA的第一个阅读框称为sur（对于src上游区域），编码富含半胱氨酸和脯氨酸残基的24千达尔顿（kDa）蛋白产物。体外分析表明24 kDa sur蛋白与膜相关。 sur蛋白的抗体在体内检测到一种24 kDa的肌肉特异性蛋白，该蛋白受到发育调节，对应于从4 KB到3 KB c-src mRNA的转换。 sur蛋白出现和pp60c-src消失的惊人动力学模式表明这两种蛋白的表达呈负相关。

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