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首页> 外文期刊>Molecular and Cellular Biology >Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.
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Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.

机译：逆转录病毒载体的基因转移仅发生在感染时正在积极复制的细胞中。

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Previous reports have shown that retrovirus infection is inhibited in nonreplicating (stationary-phase [hereafter called stationary]) cells. Infection of stationary cells was shown to occur when the cells were allowed to replicate at times up to a week after infection, suggesting that an unintegrated retrovirus could persist in a form that was competent to integrate after release of the block to replication. However, those studies were complicated by the use of replication-competent virus, which can spread in the infected cells. We have used a replication-defective retrovirus vector to compare the efficiency of gene transfer in stationary and replicating rat embryo fibroblasts. In agreement with previous results, gene transfer was inhibited 100-fold in stationary versus replicating cells. In contrast to previously reported results, the block to infection could not be relieved by stimulating stationary cells to divide at times from 6 h to 10 days after infection. Thus, for successful retroviral infection, the infected cells must be replicating at the time of infection. These results have important implications for the use of retroviral vectors for gene transfer.
机译：先前的报道表明，逆转录病毒感染在非复制性细胞（静止期[以下称为静止]）细胞中被抑制。当允许细胞在感染后最多一周的时间内复制时，就会发生固定细胞的感染，这表明未整合的逆转录病毒可以以能够释放出复制后的整合能力的形式持续存在。但是，这些研究由于使用具有复制能力的病毒而变得复杂，该病毒可以在受感染的细胞中传播。我们已经使用了复制缺陷型逆转录病毒载体来比较固定和复制大鼠胚胎成纤维细胞中基因转移的效率。与先前的结果一致，基因转移在静止细胞与复制细胞中被抑制了100倍。与先前报道的结果相反，不能通过刺激固定细胞在感染后6小时到10天之间分裂来缓解感染障碍。因此，对于成功的逆转录病毒感染，被感染的细胞必须在感染时复制。这些结果对于使用逆转录病毒载体进行基因转移具有重要意义。

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