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首页> 外文期刊>Molecular and Cellular Biology >Functional domains of a positive regulatory protein, PHO4, for transcriptional control of the phosphatase regulon in Saccharomyces cerevisiae.
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Functional domains of a positive regulatory protein, PHO4, for transcriptional control of the phosphatase regulon in Saccharomyces cerevisiae.

机译：阳性调节蛋白PHO4的功能域，用于酿酒酵母中磷酸酶调节子的转录控制。

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摘要

The PHO4 gene encodes a positive regulatory factor involved in regulating transcription of various genes in the phosphatase regulon of Saccharomyces cerevisiae. Besides its own coding region, the 1.8-kilobase PHO4 transcript contains a coding region for a mitochondrial protein which does not appear to be translated. Four functional domains were found in the PHO4 protein, which consists of 312 amino acid (aa) residues as deduced from the open reading frame of PHO4. A gel retardation assay with beta-galactosidase::PHO4 fused protein revealed that the 85-aa C terminus is the domain responsible for binding to the promoter DNA of PHO5, a gene under the control of PHO4. This region has similarities with the amphipathic helix-loop-helix motif of c-myc protein. Determination of the nucleotide sequences of four PHO4c mutant alleles and insertion and deletion analyses of PHO4 DNA indicated that a region from aa 163 to 202 is involved in interaction with a negative regulatory factor PHO80. Complementation of a pho4 null allele with the modified PHO4 DNAs suggested that the N-terminal region (1 to 109 aa), which is rich in acidic aa, is the transcriptional activation domain. The deleterious effects of various PHO4 mutations on the constitutive transcription of PHO5 in PHO4c mutant cells suggested that the region from aa 203 to 227 is involved in oligomerization of the PHO4 protein.
机译：PHO4基因编码一个正调控因子，参与调节酿酒酵母磷酸酶调节子中各种基因的转录。除了其自身的编码区之外，1.8碱基对的PHO4转录本还包含一个似乎未翻译的线粒体蛋白的编码区。在PHO4蛋白中发现了四个功能结构域，该结构域由312个氨基酸（aa）残基组成，该残基由PHO4的开放阅读框推导而来。用β-半乳糖苷酶:: PHO4融合蛋白进行的凝胶阻滞分析显示，85-aa C末端是负责与PHO5的启动子DNA结合的域，PHO5是PHO4的控制基因。该区域与c-myc蛋白的两亲性螺旋-环-螺旋基序相似。四个PHO4c突变体等位基因核苷酸序列的确定以及PHO4 DNA的插入和缺失分析表明，氨基酸163至202的区域与负调节因子PHO80相互作用。 pho4空等位基因与修饰的PHO4 DNA的互补表明，富含酸性氨基酸的N末端区域（1到109个氨基酸）是转录激活域。各种PHO4突变对PHO4c突变细胞中PHO5组成型转录的有害影响表明，氨基酸203至227的区域参与了PHO4蛋白的寡聚。

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