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首页> 外文期刊>Molecular and Cellular Biology >Morphological evidence for cyclic AMP-induced reverse transformation in vole cells infected with avian sarcoma virus.
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Morphological evidence for cyclic AMP-induced reverse transformation in vole cells infected with avian sarcoma virus.

机译：形态学证据表明，环AMP诱导的被禽肉瘤病毒感染的田鼠细胞发生反向转化。

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Normal fibroblasts of the vole displayed moderately spread or flattened, spindle-shaped, or polygonal morphologies and attached firmly to a substrate. Topographic features of these cells included sparse microvilli, ruffles, and filopodia. Microfilament bundles, intermediate filaments, and long microtubules generally parallel to each other, and the long axis of the cell or its extensions were present in the cytoplasm. Fibronectin was abundant, and fibronectin fibrils often formed junctions at the cell membrane with microfilament bundles. Transformation with avian sarcoma virus converted 90% of the cells to spheres 5 to 10 microns in diameter. In contrast to the normal vole cells, microfilament bundles were absent, microtubules were short and randomly arranged, and fibronectin was no longer visible. Exposure to dibutyryl cyclic AMP and testololactone caused a majority of the spherical cells to stretch and flatten, a process referred to as reverse transformation. Microtubules radiated out to the cell periphery and became parallel in cell extensions, while long microfilament bundles appeared in the cytoplasm. Parallel intermediate filaments were arranged throughout the cell. This ultrastructural analysis of reverse transformation in avian sarcoma virus-transformed vole cells detailed the status of the cytoskeletal system and showed agreement with earlier findings (Puck et al., J. Cell. Physiol. 107:399-412, 1981) using indirect immunofluorescence.
机译：正常的田鼠成纤维细胞显示适度散布或变平，纺锤形或多边形形态，并牢固地附着在基质上。这些细胞的地形特征包括稀疏的微绒毛，皱纹和丝状伪足。微丝束，中间丝和长微管通常彼此平行，并且细胞质的长轴或其延伸存在于细胞质中。纤连蛋白丰富，纤连蛋白原纤维经常在细胞膜上与微丝束形成连接。用禽肉瘤病毒转化将90％的细胞转化为直径5至10微米的球体。与正常的田鼠细胞相反，缺少微丝束，微管短且随机排列，并且纤连蛋白不再可见。暴露于二丁酰基环AMP和睾丸内酯导致大多数球形细胞拉伸和扁平化，这一过程称为反向转化。微管辐射到细胞外围并在细胞延伸中变得平行，而长的微丝束出现在细胞质中。平行的中间丝排列在整个电池中。在禽肉瘤病毒转化的田鼠细胞中进行反向转化的超微结构分析详细描述了细胞骨架系统的状态，并显示了与使用间接免疫荧光技术的早期发现（Puck等人，J。Cell。Physiol。107：399-412，1981）相一致的结果。 。

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