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首页> 外文期刊>Molecular and Cellular Biology >Newly formed mRNA lacking polyadenylic acid enters the cytoplasm and the polyribosomes but has a shorter half-life in the absence of polyadenylic acid.
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Newly formed mRNA lacking polyadenylic acid enters the cytoplasm and the polyribosomes but has a shorter half-life in the absence of polyadenylic acid.

机译：缺乏聚腺苷酸的新形成的mRNA进入细胞质和多核糖体，但是在不存在聚腺苷酸的情况下具有较短的半衰期。

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摘要

Labeled adenovirus type 2 nuclear RNA molecules from cells treated with 3'-deoxyadenosine (3'dA) were earlier reported to lack polyadenylic acid [poly(A)], but to be correctly spliced in the nucleus (M. Zeevi et al., Cell 26:39-46, 1981). We have now found that the shortened mRNA molecules, lacking poly(A), can also be found in the cytoplasm of 3'dA-treated cells in association with the polyribosomes. In addition, the accumulation of labeled, nuclear adenovirus-specific RNA complementary to early regions 1a, 1b, and 2 of the adenovirus genome was approximately equal in 3'dA-treated and control cells. At the initial appearance of newly labeled adenovirus type 2 RNA (10 min) in the cytoplasm, there was one-half as much labeled RNA in 3'dA-treated cells as in the control. However, control cells accumulated additional mRNA in the cytoplasm very rapidly in the first 40 min of labeling, whereas the 3'dA-treated cells did not. Therefore, it appears that the correctly spliced, poly(A)- mRNA molecules that are labeled in the presence of 3'dA can be transported from the nucleus with nearly the same frequency and the same exit time as in control cells and can be translated in the cytoplasm but have a much shorter half-life than the poly(A)+ mRNA molecules from control infected cells. From these results it is suggested that the role of poly(A) may be entirely to increase the longevity of cytoplasmic mRNA.
机译：据报道，用3'-脱氧腺苷（3'dA）处理过的细胞中标记的2型腺病毒核RNA分子缺乏聚腺苷酸[poly（A）]，但已正确地剪接在细胞核中（M. Zeevi等， Cell 26：39-46，1981）。现在我们已经发现，缺乏poly（A）的缩短的mRNA分子也可以在与多核糖体结合的3'dA处理细胞的细胞质中发现。另外，与腺病毒基因组的早期区域1a，1b和2互补的标记核腺病毒特异性RNA的积累在经过3'dA处理的细胞和对照细胞中大约相等。在细胞质中新标记的2型腺病毒RNA初次出现（10分钟）时，经3'dA处理的细胞中的标记RNA数量是对照组的一半。但是，对照细胞在标记的前40分钟内非常迅速地在细胞质中积累了额外的mRNA，而3'dA处理的细胞却没有。因此，看来在3'dA存在下标记的正确剪接的poly（A）-mRNA分子可以以与对照细胞几乎相同的频率和相同的退出时间从细胞核中转运出来并可以翻译但其半衰期比对照感染细胞的poly（A）+ mRNA分子短得多。从这些结果表明，poly（A）的作用可能完全是增加细胞质mRNA的寿命。

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