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首页> 外文期刊>Molecular and Cellular Biology >Induction of a step in carcinogenesis that is normally associated with mutagenesis by nonmutagenic concentrations of 5-azacytidine.
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Induction of a step in carcinogenesis that is normally associated with mutagenesis by nonmutagenic concentrations of 5-azacytidine.

机译：非致癌浓度的5-氮杂胞苷诱导通常与致突变有关的致癌步骤。

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The permanent cell line BHK-21/cl 13 can be transformed by mutagenic carcinogens as the result of the induction of a recessive somatic mutation. Yet when these cells were treated with 5-azacytidine under conditions in which no mutants resistant to either ouabain or 6-thioguanine could be detected, they were transformed efficiently. These transformants were induced, not selected. 6-Azacytidine was ineffective at transforming BHK cells; 2'-deoxy-5-azacytidine was exceptionally effective. When tested by cell fusion, transformants induced by 5-azacytidine fell into the same complementation group as those induced by highly mutagenic carcinogens, but they were phenotypically distinct in that they were unstable during prolonged passage and rarely displayed the temperature-limited phenotypes so common among BHK transformants induced by strongly mutagenic carcinogens. These results raise the possibility that a cell can be induced by either genetic or epigenetic means to traverse the same single step in carcinogenesis.
机译：由于隐性体细胞突变的诱导，永久细胞系BHK-21 / cl 13可以被诱变致癌物转化。然而，当在无法检测到对哇巴因或6-硫代鸟嘌呤有抗性的突变体的条件下，用5-氮胞苷处理这些细胞时，它们被有效地转化了。这些转化子被诱导，未被选择。 6-氮杂胞苷对转化BHK细胞无效。 2'-脱氧-5-氮胞苷特别有效。当通过细胞融合测试时，由5-氮杂胞苷诱导的转化子与高度致突变性致癌物诱导的转化子属于同一互补组，但是它们在表型上不同，因为它们在长时间传代过程中不稳定，并且很少显示出温度受限的表型。由强烈诱变的致癌物诱导的BHK转化子。这些结果增加了可以通过遗传或表观遗传学手段诱导细胞穿越癌变过程中同一步骤的可能性。

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