Immunological and genetic characterization of 2-deoxygalactose-resistant, galactokinase-deficient mutants of Chinese hamster cells: evidence for structural mutations at the galK locus.

B Talbot; C A de Souza; D Banville; J P Thirion

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Immunological and genetic characterization of 2-deoxygalactose-resistant, galactokinase-deficient mutants of Chinese hamster cells: evidence for structural mutations at the galK locus.

机译：中国仓鼠细胞的2-脱氧半乳糖耐药，半乳糖激酶缺陷型突变体的免疫学和遗传学特征：galK基因座结构突变的证据。

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Ten independent mutants resistant to 2-deoxygalactose and without any detectable galactokinase activity (null-galactokinase mutations) were isolated from mutagenized Chinese hamster somatic cells. They were analyzed for the presence of serologically cross-reacting material (CRM) with antiserum generated against highly purified Chinese hamster galactokinase. All 10 mutants contain cross-reacting material (i.e., were CRM+), indicating that all the mutations affect the correct expression of a product of the galactokinase structural gene. Complementation analysis among them shows that the 10 mutations fall in one functional genetic unit.

机译：从诱变的中国仓鼠体细胞中分离出十个对2-脱氧半乳糖有抗性并且没有任何可检测的半乳糖激酶活性（空半乳糖激酶突变）的独立突变体。分析了它们的血清学交叉反应物质（CRM），以及针对高度纯化的中国仓鼠半乳糖激酶产生的抗血清。所有10个突变体均包含交叉反应物质（即CRM +），表明所有突变均会影响半乳糖激酶结构基因产物的正确表达。其中的互补分析表明，这10个突变属于一个功能遗传单位。

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《Molecular and Cellular Biology》 |1984年第11期|共7页
作者
B Talbot; C A de Souza; D Banville; J P Thirion;
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中图分类细胞生物学;
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6. Immunological and genetic characterization of 2-deoxygalactose-resistant galactokinase-deficient mutants of Chinese hamster cells: evidence for structural mutations at the galK locus. [O] . B Talbot, C A de Souza, D Banville, 1984

机译：中国仓鼠细胞的2-脱氧半乳糖耐药半乳糖激酶缺陷型突变体的免疫学和遗传学特征：galK基因座结构突变的证据。
7. Immunological and genetic characterization of 2-deoxygalactose-resistant, galactokinase-deficient mutants of Chinese hamster cells: evidence for structural mutations at the galK locus. [O] . Talbot, B, de Souza, C A, Banville, D, 1984

机译：中国仓鼠细胞的2-脱氧半乳糖耐药，半乳糖激酶缺陷型突变体的免疫学和遗传学特征：galK基因座结构突变的证据。
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机译：高原期中国仓鼠卵巢细胞HGpRT位点X射线诱导突变的分子特征：剂量，剂量分级和延迟电镀的影响。

Immunological and genetic characterization of 2-deoxygalactose-resistant, galactokinase-deficient mutants of Chinese hamster cells: evidence for structural mutations at the galK locus.

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