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首页> 外文期刊>Molecular and Cellular Biology >Cloning and mapping of Saccharomyces cerevisiae photoreactivation gene PHR1.
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Cloning and mapping of Saccharomyces cerevisiae photoreactivation gene PHR1.

机译：酿酒酵母光活化基因PHR1的克隆和定位。

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摘要

The yeast Saccharomyces cerevisiae, like most organisms, is able to directly repair pyrimidine dimers by using a photoreactivating enzyme and visible light. Cells carrying the phr1 mutation were shown previously to be unable to photoreactivate dimers, but neither the map position nor the primary gene product of the PHR1 gene has been determined. We have cloned this gene and determined its map position. A plasmid containing a 6.4-kilobase yeast DNA insert has been isolated and shown to restore photoreactivation in a phr1 strain. A 3.1-kilobase subclone has also been shown to complement phr1. The original plasmid was targeted to integrate into chromosomal DNA at a site homologous to the insert by cutting within the insert. Two of these integrants have been mapped on the right arm of chromosome XV; the integrants have been further mapped at ca. 13 centimorgans from prt1. It has also been independently determined that phr1 maps at this location. Thus, we have determined the map position of PHR1 and also have shown that the plasmid contains PHR1 rather than a suppressor of the phr1 mutation.
机译：像大多数生物一样，酵母酿酒酵母能够通过使用光活化酶和可见光直接修复嘧啶二聚体。先前已证明携带phr1突变的细胞无法光激活二聚体，但尚未确定PHR1基因的图谱位置或一级基因产物。我们已经克隆了该基因并确定了它的图谱位置。含有6.4碱基碱基的酵母DNA插入片段的质粒已被分离出来，显示可在phr1菌株中恢复光活化。还显示出一个3.1千碱基的亚克隆可补充phr1。通过在插入物中切割，将原始质粒靶向整合到与插入物同源的位点的染色体DNA中。这些整合物中的两个已被映射到XV染色体的右臂上。积分已进一步映射到约。来自prt1的13厘摩。还独立确定phr1映射在此位置。因此，我们确定了PHR1的图谱位置，还表明该质粒含有PHR1，而不是phr1突变的抑制剂。

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