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首页> 外文期刊>Molecular and Cellular Biology >E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.
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E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.

机译：大肠杆菌生产的E1A 13S和12S mRNA产品均起着核定位转录激活剂的作用，但不直接结合DNA。

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摘要

We previously purified and characterized functionally the Escherichia coli-expressed product of the human subgroup C adenovirus E1A 13S mRNA (B. Ferguson, N. Jones, J. Richter, and M. Rosenberg, Science 224:1343-1346, 1984; B. Krippl, B. Ferguson, M. Rosenberg, and H. Westphal, Proc. Natl. Acad. Sci. USA 81:6988-6992, 1984). We have now expressed in E. coli and purified the protein product encoded by the human subgroup C adenovirus E1A 12S mRNA and have compared the functional properties of this protein with those of the E1A 13S mRNA product. Using microinjection techniques to introduce these proteins into mammalian cells, we found that the E1A 12S mRNA product, like the 13S mRNA product, localized rapidly to the cell nucleus and induced adenovirus gene expression. Although both E1A gene products localized to the nucleus and stimulated adenovirus gene transcription, these proteins did not directly bind to DNA under conditions in which a known DNA-binding protein, the human c-myc gene product, bound DNA efficiently. Thus, the E1A and myc gene products, which have been related both structurally and functionally, exhibit distinctly different biochemical properties.
机译：我们先前已纯化并在功能上纯化了人类亚组C腺病毒E1A 13S mRNA的大肠杆菌表达产物（B.弗格森，N。琼斯，J。里希特和M.罗森伯格，《科学》 224：1343-1346，1984; B。 Krippl，B.Ferguson，M.Rosenberg和H.Westphal，Proc.Natl.Acad.Sci.USA 81：6988-6992，1984）。现在，我们已经在大肠杆菌中表达并纯化了由人类亚组C腺病毒E1A 12S mRNA编码的蛋白质产物，并将该蛋白质的功能特性与E1A 13S mRNA产物的功能特性进行了比较。使用显微注射技术将这些蛋白质引入哺乳动物细胞，我们发现E1A 12S mRNA产品（如13S mRNA产品）迅速定位到细胞核并诱导了腺病毒基因表达。尽管两种E1A基因产物均定位于核并刺激了腺病毒基因转录，但在已知的DNA结合蛋白（人c-myc基因产物）有效结合DNA的条件下，这些蛋白并未直接与DNA结合。因此，已经在结构和功能上相关的E1A和myc基因产物表现出明显不同的生化特性。

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