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首页> 外文期刊>Molecular and Cellular Biology >Proliferative response and oncogene expression induced by epidermal growth factor in EL2 rat fibroblasts.
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Proliferative response and oncogene expression induced by epidermal growth factor in EL2 rat fibroblasts.

机译：表皮生长因子在EL2大鼠成纤维细胞中诱导的增殖反应和癌基因表达。

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摘要

Extensive evidence supports a two-step model for the control of fibroblast growth, which includes first the action of a competence factor (e.g., platelet-derived growth factor) followed by the stimulus of a progression factor (e.g., epidermal growth factor [EGF]). We investigated whether this model may be applied to the euploid EL2 fibroblast line recently isolated from rat embryos (E. Liboi, M. Caruso, and C. Basilico, Mol. Cell. Biol. 4:2925-2928, 1984). Our results clearly show that EGF alone leads EL2 cells to proliferate in serum-free conditions at a rate corresponding to 50 to 60% of that observed in the presence of 10% calf serum. It is of interest that, when resting EL2 cells were exposed to EGF, transcription of both c-myc and c-fos was markedly induced. Altogether, these observations suggest that, in contrast with the model of fibroblast growth mentioned above, EL2 cells require the presence of a single growth factor (EGF) for induction of DNA synthesis, and the expression of myc and fos proto-oncogenes may represent an obligatory step in the pathway of commitment of EL2 cells to proliferation. In addition, we showed that EGF may induce EL2 cells to acquire some properties of transformed cells, such as growth in agar and loss of contact inhibition. This suggests that the particular response to EGF of the EL2 line may be strictly connected with the expression of a transformed phenotype.
机译：大量证据支持控制成纤维细胞生长的两步模型，该模型首先包括能力因子（例如血小板衍生的生长因子）的作用，其次是对进展因子（例如表皮生长因子[EGF]）的刺激。 ）。我们研究了该模型是否可以应用于最近从大鼠胚胎中分离的整倍体EL2成纤维细胞系（E.Liboi，M.Caruso，和C.Basilico，Mol.Cell.Biol.4：2925-2928，1984）。我们的结果清楚地表明，单独的EGF可使EL2细胞在无血清条件下增殖，其速率相当于在10％小牛血清中观察到的速率的50％至60％。令人感兴趣的是，当静止的EL2细胞暴露于EGF时，c-myc和c-fos的转录均被明显诱导。总之，这些观察结果表明，与上述成纤维细胞生长模型相反，EL2细胞需要存在单个生长因子（EGF）才能诱导DNA合成，而myc和fos原癌基因的表达可能代表了EL2细胞承诺增殖途径中的强制性步骤。此外，我们证明了EGF可能诱导EL2细胞获得转化细胞的某些特性，例如琼脂的生长和接触抑制的丧失。这表明EL2细胞系对EGF的特殊反应可能与转化表型的表达紧密相关。

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