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Elements involved in oxygen regulation of the Saccharomyces cerevisiae CYC7 gene.

机译：参与酿酒酵母CYC7基因氧调控的元素。

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摘要

The CYC7 gene of Saccharomyces cerevisiae encodes the minor species, iso-2, of the cytochrome c protein. Its expression is governed by two regulatory sequences upstream from the gene: a positive site which stimulates transcription 240 base pairs 5' from the protein-coding sequence (-240) and a negative site which inhibits transcription at -300. In this study, the nature of the positive site and its relationship to the negative site has been investigated. Expression of the CYC7 gene is weakly inducible by oxygen. This effect was greatly enhanced by the semidominant CYP1-16 mutation in the trans-acting gene CYP1. The weak oxygen regulation in wild-type cells and the enhanced induction in CYP1-16 mutants were found to be mediated through the positive site. A mutational analysis of this site implicated at least part of a tandem, direct repeat of 9 base pairs as essential for the functioning of this site. The relationship between the positive and negative sites was investigated by comparing the expression of the intact gene with that of derivatives lacking either one or the other site. The expression of the gene containing only the negative site was actually stimulated anaerobically, while the gene containing the positive site alone, although having higher expression aerobically than anaerobically, had higher anaerobic expression than did the intact gene. Thus, it appeared that the combination of the positive and negative sites suppressed anaerobic expression. A model which attempts to explain these properties of the two sites and account for the regulation of the expression of the intact gene is presented.
机译：酿酒酵母的CYC7基因编码细胞色素c蛋白的次要物种iso-2。它的表达由基因上游的两个调节序列控制：一个正向位点刺激一个从蛋白质编码序列（-240）开始的240个碱基对的5'转录;一个负向位点抑制-300位的转录。在这项研究中，已经研究了阳性位点的性质及其与阴性位点的关系。 CYC7基因的表达可被氧气弱诱导。反式作用基因CYP1中的半显性CYP1-16突变大大增强了该作用。发现通过阳性位点介导野生型细胞中的弱氧调节和CYP1-16突变体中增强的诱导。此位点的突变分析表明，串联串联，直接重复9个碱基对的至少一部分是该位点功能必不可少的。通过比较完整基因的表达与缺乏一个或另一个位点的衍生物的表达，研究了正负位点之间的关系。实际上仅厌氧地刺激仅包含阴性位点的基因的表达，而单独的仅含有阳性位点的基因尽管需氧表达高于厌氧表达，但其厌氧表达高于完整基因。因此，似乎正负位点的组合抑制了厌氧表达。提出了一个试图解释这两个位点的这些特性并解释完整基因表达调控的模型。

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