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首页> 外文期刊>Molecular and Cellular Biology >Identification of an essential Schizosaccharomyces pombe RNA homologous to the 7SL component of signal recognition particle.
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Identification of an essential Schizosaccharomyces pombe RNA homologous to the 7SL component of signal recognition particle.

机译：鉴定与信号识别颗粒的7SL组分同源的必要的粟酒裂殖酵母RNA。

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We have cloned the gene encoding a novel small cytoplasmic RNA from the fission yeast Schizosaccharomyces pombe. Four lines of evidence support the idea that this RNA is a homolog of the 7SL RNA component of mammalian signal recognition particle (SRP), which targets presecretory proteins to the endoplasmic reticulum membrane. First, it shares limited but significant primary sequence homology with previously identified 7SL RNAs and can be folded into a similar secondary structure. Second, it possesses the 5' triphosphate characteristic of unprocessed RNA polymerase III transcripts, and moreover, it is the only fission yeast RNA in this size range with such a terminus. Third, its behavior in cell fractionation experiments suggests that it is part of a small ribonucleoprotein which forms salt-labile contacts with larger structures. Fourth, the particle containing S. pombe 7SL RNA resembles mammalian SRP in both size (11S) and affinity for DEAE-Sepharose. Disruption of the single-copy gene, designated slr1+, reveals that the RNA is indispensable for growth in fission yeast. This result is not surprising, since secretion is an essential cellular process.
机译：我们已经从裂殖酵母粟酒裂殖酵母克隆了编码新型小细胞质RNA的基因。有四个证据支持这一想法，即该RNA是哺乳动物信号识别颗粒（SRP）的7SL RNA组分的同源物，该组分将分泌前的蛋白质靶向内质网膜。首先，它与先前鉴定的7SL RNA具有有限但重要的一级序列同源性，可以折叠成相似的二级结构。其次，它具有未加工的RNA聚合酶III转录本的5'三磷酸特征，而且，它是在该大小范围内具有这种末端的唯一裂变酵母RNA。第三，其在细胞分离实验中的行为表明它是小的核糖核蛋白的一部分，可与较大的结构形成盐不稳定的接触。第四，含有粟酒裂殖酵母7SL RNA的颗粒在大小（11S）和对DEAE-Sepharose的亲和力上均类似于哺乳动物SRP。单拷贝基因（称为slr1 +）的破坏表明，RNA在裂变酵母中的生长不可或缺。这个结果不足为奇，因为分泌是必不可少的细胞过程。

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