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首页> 外文期刊>Molecular and Cellular Biology >Isolation of a yeast gene involved in species-specific pre-tRNA processing.
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Isolation of a yeast gene involved in species-specific pre-tRNA processing.

机译：分离涉及物种特异性pre-tRNA加工的酵母基因。

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To identify genes involved in pre-tRNA processing, we searched for yeast DNA sequences that specifically enhanced the expression of the SUP4(G37) gene. The SUP4(G37) gene possesses a point mutation at position 37 of suppressor tRNA(Tyr). This lesion results in a reduced rate of pre-tRNA splicing and a decreased level of nonsense suppression. A SUP4(G37) strain was transformed with a yeast genomic library, and the transformants were screened for increased suppressor activity. One transformant contained a plasmid that encoded an unessential gene, STP1, that in multiple copies enhanced the suppression of SUP4(G37) and caused increased production of mature SUP4(G37) product. Disruption of the genomic copy of STP1 resulted in a reduced efficiency of SUP4-mediated suppression and the accumulation of pre-tRNAs. Not all intron-containing pre-tRNAs were affected by the stp1-disruption. At least five of the nine families of pre-tRNAs were affected. Two other species, pre-tRNA(Ile) and pre-tRNA(3Leu), were not. We propose that STP1 encodes a tRNA species-specific product that functions as a helper for pre-tRNA splicing. The STP1 product may interact with pre-tRNAs to generate a structure that is efficiently recognized by splicing machinery.
机译：为了鉴定涉及前tRNA加工的基因，我们搜索了特异性增强SUP4（G37）基因表达的酵母DNA序列。 SUP4（G37）基因在抑制性tRNA（Tyr）的37位具有点突变。这种病变导致tRNA前剪接的速率降低，并且废话抑制水平降低。用酵母基因组文库转化SUP4（G37）菌株，并针对转化子的抑制活性进行筛选。一个转化体包含一个编码非必需基因STP1的质粒，该质粒以多个拷贝增强了对SUP4（G37）的抑制作用，并导致增加了成熟SUP4（G37）产品的产量。 STP1基因组拷贝的破坏导致SUP4介导的抑制效率降低以及pre-tRNA的积累。并非所有含内含子的pre-tRNA都受到stp1干扰的影响。 9个pre-tRNA家族中至少有5个受到了影响。其他两个物种不是pre-tRNA（Ile）和pre-tRNA（3Leu）。我们建议STP1编码tRNA物种特异性的产品，其功能可作为pre-tRNA剪接的辅助工具。 STP1产品可能与pre-tRNA相互作用，产生一种可以被剪接机制有效识别的结构。

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