Enhanced transcription of the 78,000-dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1).

T Nakaki; R J Deans; A S Lee

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Enhanced transcription of the 78,000-dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1).

机译：非分泌性B细胞骨髓瘤系（NS-1）中78,000道尔顿葡萄糖调节蛋白（GRP78）基因的转录增强，以及GRP78与免疫球蛋白轻链的关联。

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The 78,000-dalton glucose-regulated protein (GRP78) is a stress-inducible protein localized in the endoplasmic reticulum. It has been identified as the immunoglobulin heavy-chain-binding protein. We report here a high level of GRP78 expression in a B-cell myeloma line, NS-1, which produces only kappa light-chain proteins but is unable to secrete them. GRP78 transcription was enhanced in NS-1 cells, resulting in higher levels of GRP78 mRNA and protein than in non-immunoglobulin-producing cells. Furthermore, the nonsecreted light chains in NS-1 cells were found in specific association with GRP78. We hypothesize that in nonsecreting lymphoid cells, the presence of free, unassembled light chains in the endoplasmic reticulum could result in increased transcription of the GRP78 gene and that GRP78 can also bind to immunoglobulin light chains.

机译：78,000道尔顿的葡萄糖调节蛋白（GRP78）是位于内质网中的应激诱导蛋白。已经鉴定为免疫球蛋白重链结合蛋白。我们在这里报告了B细胞骨髓瘤系NS-1中的高水平GRP78表达，该系仅产生kappa轻链蛋白，但无法分泌它们。与非免疫球蛋白生产细胞相比，NS-1细胞中的GRP78转录增强，导致GRP78 mRNA和蛋白水平更高。此外，发现NS-1细胞中的非分泌轻链与GRP78特异性相关。我们假设在非分泌性淋巴样细胞中，内质网中游离，未组装的轻链的存在可能导致GRP78基因的转录增加，并且GRP78也可以与免疫球蛋白轻链结合。

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《Molecular and Cellular Biology》 |1989年第5期|共6页
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T Nakaki; R J Deans; A S Lee;
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1. Identity of the immunoglobulin heavy-chain-binding protein with the 78,000-dalton glucose-regulated protein and the role of posttranslational modifications in its binding function. [J] . L M Hendershot, J Ting, A S Lee Molecular and Cellular Biology . 1988,第10期

机译：免疫球蛋白重链结合蛋白与78,000道尔顿葡萄糖调节蛋白的同一性以及翻译后修饰在其结合功能中的作用。
2. Cloning of a functional Burkitt's lymphoma polypeptide-binding protein/78 kDa glucose-regulated protein (BiP/GRP78) gene promoter by the polymerase chain reaction, and its interaction with inducible cellular factors [J] . C C K Chao, L K Chen, S Lin-Chao, The biochemical journal . 1992,第2期

机译：通过聚合酶链反应克隆功能性伯基特氏淋巴瘤多肽结合蛋白/ 78 kDa葡萄糖调节蛋白（BiP / GRP78）基因启动子及其与诱导性细胞因子的相互作用
3. Silencing of Glucose-Regulated Protein 78 (GRP78) Enhances Cell Migration Through the Upregulation of Vimentin in Hepatocellular Carcinoma Cells [J] . Po-Li Wei, Li-Jen Kuo, Weu Wang, Annals of Surgical Oncology . 2012,第s3期

机译：沉默葡萄糖调节蛋白78（GRP78）通过肝细胞癌细胞中波形蛋白的上调增强细胞迁移。
4. Association of glucose-regulated protein 78 (GRP78) with ovarian cancer development and progression. [D] . Alongkronrusmee, Doungkamol. 2013

机译：葡萄糖调节蛋白78（GRP78）与卵巢癌发生发展的关系。
5. Enhanced transcription of the 78000-dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1). [O] . T Nakaki, R J Deans, A S Lee 1989

机译：非分泌性B细胞骨髓瘤细胞系（NS-1）中78000道尔顿葡萄糖调节蛋白（GRP78）基因的转录增强以及GRP78与免疫球蛋白轻链的关联。
6. Enhanced transcription of the 78,000-dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1). [O] . Nakaki, T, Deans, R J, Lee, A S 1989

机译：非分泌性B细胞骨髓瘤细胞系（NS-1）中78,000道尔顿葡萄糖调节蛋白（GRP78）基因的转录增强以及GRP78与免疫球蛋白轻链的关联。

Enhanced transcription of the 78,000-dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1).

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