...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Regulation of glucose transporters in LLC-PK1 cells: effects of D-glucose and monosaccharides.
【24h】

Regulation of glucose transporters in LLC-PK1 cells: effects of D-glucose and monosaccharides.

机译：LLC-PK1细胞中葡萄糖转运蛋白的调节：D-葡萄糖和单糖的作用。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Regulation of D-glucose transport in the porcine kidney epithelial cell line LLC-PK1 was examined. To identify the sodium-coupled glucose transporter (SGLT), we cloned and sequenced several partial cDNAs homologous to SGLT1 from rabbit small intestine (M. A. Hediger, M. J. Coady, T. S. Ikeda, and E. M. Wright, Nature (London) 330:379-381, 1987). The extensive homology of the two sequences leads us to suggest that the high-affinity SGLT expressed by LLC-PK1 cells is SGLT1. SGLT1 mRNA levels were highest when the D-glucose concentration in the culture medium was 5 to 10 mM. Addition of D-mannose or D-fructose, but not D-galactose, in the presence of 5 mM D-glucose suppressed SGLT1 mRNA levels. SGLT1 activity, measured by methyl alpha-D-glucopyranoside uptake, paralleled message levels except in cultures containing D-galactose. Therefore, SGLT1 gene expression may respond either to the cellular energy status or to the concentration of a hexose metabolite(s). By isolating several cDNAs homologous to rat GLUT-1, we identified the facilitated glucose transporter in LLC-PK1 cells as the erythroid/brain type GLUT-1. High-stringency hybridization of a single mRNA transcript to the rat GLUT-1 cDNA probe and failure to observe additional transcripts hybridizing either to GLUT-1 or to GLUT-2 probes at low stringency provide evidence that GLUT-1 is the major facilitated glucose transporter in this cell line. LLC-PK1 GLUT-1 mRNAs were highest at medium D-glucose concentrations of less than or equal to 2 mM. D-Fructose, D-mannose, and to a lesser extent D-galactose all suppressed GLUT-1 mRNA levels. Since the pattern of SGLT1 and GLUT-1 expression differed, particularly in low D-glucose or in the presence of D-galactose, we suggest that the two transporters are regulated independently.
机译：检查了猪肾上皮细胞系LLC-PK1中D-葡萄糖运输的调节。为了鉴定钠耦合的葡萄糖转运蛋白（SGLT），我们从兔小肠中克隆了一些与SGLT1同源的部分cDNA（MA Hediger，MJ Coady，TS Ikeda和EM Wright，Nature（伦敦）330：379-381， 1987）。这两个序列的广泛同源性使我们认为LLC-PK1细胞表达的高亲和力SGLT是SGLT1。当培养基中D-葡萄糖的浓度为5至10 mM时，SGLT1 mRNA水平最高。在5 mM D-葡萄糖存在下，添加D-甘露糖或D-果糖而不添加D-半乳糖会抑制SGLT1 mRNA水平。通过甲基α-D-吡喃葡萄糖苷的摄取测量的SGLT1活性与含有D-半乳糖的培养液中的信息水平平行。因此，SGLT1基因表达可能会响应细胞的能量状态或己糖代谢产物的浓度。通过分离与大鼠GLUT-1同源的几个cDNA，我们鉴定了LLC-PK1细胞中促进的葡萄糖转运蛋白为类红/脑型GLUT-1。单个mRNA转录物与大鼠GLUT-1 cDNA探针的高严格杂交，以及未能观察到以低严格性与GLUT-1或GLUT-2探针杂交的其他转录本，提供了GLUT-1是主要的促进葡萄糖转运蛋白的证据在这个细胞系中LLC-PK1 GLUT-1 mRNA在中等D-葡萄糖浓度小于或等于2 mM时最高。 D-果糖，D-甘露糖和较小程度的D-半乳糖均抑制GLUT-1 mRNA水平。由于SGLT1和GLUT-1的表达模式有所不同，特别是在低D-葡萄糖或D-半乳糖存在的情况下，我们建议这两个转运蛋白是独立调节的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号