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首页> 外文期刊>Molecular and Cellular Biology >Platelet-derived growth factor increases the in vivo activity of phospholipase C-gamma 1 and phospholipase C-gamma 2.
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Platelet-derived growth factor increases the in vivo activity of phospholipase C-gamma 1 and phospholipase C-gamma 2.

机译：血小板衍生的生长因子可增加磷脂酶C-γ1和磷脂酶C-γ2的体内活性。

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摘要

Upon binding to its cell surface receptor, platelet-derived growth factor (PDGF) causes the tyrosine phosphorylation of phospholipase C-gamma 1 (PLC-gamma 1) and stimulates the production of diacylglycerol and inositol 1,4,5-triphosphate. We showed that following stimulation by PDGF, rat-2 cells overexpressing PLC-gamma 1 display an increase in the levels of both tyrosine-phosphorylated PLC-gamma 1 and inositol phosphates compared with the parental rat-2 cells. This increased responsiveness to PDGF is a direct effect of PLC-gamma 1 overexpression, as a cell line expressing similar levels of an enzymatically inactive point mutant of PLC-gamma 1, PLC-gamma 1 335Q, did not show elevated inositol phosphate production in response to PDGF. Hematopoietic cells express PLC-gamma 2, a PLC isoform that is closely related to PLC-gamma 1. When rat-2 cells overexpressing PLC-gamma 2 were treated with PDGF, an increase in both the tyrosine phosphorylation and the in vivo activity of PLC-gamma 2 was observed. Aluminum fluoride (AIF4-), a universal activator of PLC linked to G-proteins, did not produce an increase in the levels of inositol phosphates in either of the overexpressing cell lines compared with parental rat-2 cells, demonstrating that PLC-gamma isoforms respond specifically to a receptor with tyrosine kinase activity.
机译：血小板衍生生长因子（PDGF）与其细胞表面受体结合后，引起磷脂酶C-γ1（PLC-γ1）的酪氨酸磷酸化，并刺激产生二酰基甘油和肌醇1,4,5-三磷酸。我们显示，PDGF刺激后，与亲本大鼠2细胞相比，过表达PLC-γ1的大鼠2细胞显示酪氨酸磷酸化的PLC-γ1和肌醇磷酸酯水平都增加。对PDGF的反应性增强是PLC-γ1过表达的直接影响，因为表达类似水平的PLC-γ1，PLC-γ1 335Q的酶失活点突变体的细胞系在反应中未显示出升高的肌醇磷酸生成到PDGF。造血细胞表达PLC-γ2（一种与PLC-γ1密切相关的PLC异构体）。用PDGF处理过表达PLC-γ2的rat-2细胞时，酪氨酸磷酸化和PLC的体内活性均增加观察到-γ2。与亲本大鼠2细胞相比，氟化铝（AIF4-）是与G蛋白连接的PLC的通用激活剂，在两种过表达的细胞系中均未产生肌醇磷酸水平的增加，这表明PLC-γ亚型对具有酪氨酸激酶活性的受体有特异性反应。

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