Identification of a specific exon sequence that is a major determinant in the selection between a natural and a cryptic 5' splice site.

L Domenjoud; H Gallinaro; L Kister; S Meyer; M Jacob

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Identification of a specific exon sequence that is a major determinant in the selection between a natural and a cryptic 5' splice site.

机译：鉴定特定外显子序列，该序列是在天然5'和隐性5'剪接位点之间进行选择的主要决定因素。

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The first intron of the early region 3 from adenovirus type 2 contains a cryptic 5' splice site, Dcr1, 74 nucleotides downstream from the natural site D1. The cryptic site can be activated when the natural site is inactivated by mutagenesis. To investigate the basis for selection between a natural and a cryptic 5' splice site, we searched for cis-acting elements responsible for the exclusive selection of the natural site. We show that both the relative intrinsic strength of the sites and the sequence context affect the selection. A 120-nucleotide segment located at the 3' end of exon 1 enhances splicing at the proximal site D1; in its absence the two sites are used according to their strength. Thus, three cis-acting elements are involved in the silencing of the cryptic site: the sequence of D1, the sequence of Dcr1, and an upstream exonic sequence. We show that the exonic element folds, in solution, into a 113-nucleotide-long stem-loop structure. We propose that this potential stem-loop structure which is located 6 nucleotides upstream of the exon 1-intron junction is responsible for the preferential use of the natural 5' splice site.

机译：来自2型腺病毒的早期区域3的第一个内含子包含一个隐性5'剪接位点Dcr1，即天然位点D1下游的74个核苷酸。当通过诱变使天然位点失活时，可以激活隐蔽位点。为了研究在天然和隐蔽的5'剪接位点之间进行选择的基础，我们搜索了负责天然位点排他性选择的顺式作用元件。我们表明站点的相对内在强度和序列上下文都影响选择。位于外显子1 3'端的120个核苷酸的片段增强了近端位点D1的剪接;在没有它的情况下，根据强度使用两个部位。因此，沉默位点的沉默涉及三个顺式作用元件：D1序列，Dcr1序列和上游外显子序列。我们显示外显子元素在溶液中折叠成113个核苷酸长的茎环结构。我们提出，位于外显子1-内含子连接上游6个核苷酸的潜在茎-环结构负责优先使用天然5'剪接位点。

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《Molecular and Cellular Biology》 |1991年第9期|共10页
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L Domenjoud; H Gallinaro; L Kister; S Meyer; M Jacob;
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1. Activation of cryptic splice sites is a frequent splicing defect mechanism caused by mutations in exon and intron sequences of the OPA1 gene. [J] . Schimpf S, Schaich S, Wissinger B Human Genetics . 2006,第6期

机译：隐蔽剪接位点的激活是由OPA1基因的外显子和内含子序列突变引起的常见剪接缺陷机制。
2. Alternative splicing of a human alpha-tropomyosin muscle-specific exon: identification of determining sequences. [J] . I R Graham, M Hamshere, I C Eperon Molecular and Cellular Biology . 1992,第9期

机译：人α-原肌球蛋白肌肉特异性外显子的选择性剪接：确定序列的鉴定。
3. Cryptic splice site usage in exon 7 of the human fibrinogen Bbeta-chain gene is regulated by a naturally silent SF2/ASF binding site within this exon. [J] . Spena S, Tenchini ML, Buratti E RNA . 2006,第6期

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4. The Identification of Human Cryptic Exons Based on SVM [C] . Gang Su, Ying-Fei Sun, Jun Li Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009 . 2009

机译：基于支持向量机的人类隐性外显子识别
5. Selection of the exon junction complex deposition site during pre-mRNA splicing. [D] . Mishler, Dennis Michael. 2009

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6. Identification of a specific exon sequence that is a major determinant in the selection between a natural and a cryptic 5 splice site. [O] . L Domenjoud, H Gallinaro, L Kister, 1991

机译：鉴定特定外显子序列该序列是在天然5和隐性5剪接位点之间进行选择的主要决定因素。
7. Identification of a specific exon sequence that is a major determinant in the selection between a natural and a cryptic 5' splice site. [O] . Domenjoud, L, Gallinaro, H, Kister, L, 1991

机译：鉴定特定外显子序列，该序列是在天然5'和隐性5'剪接位点之间进行选择的主要决定因素。

Identification of a specific exon sequence that is a major determinant in the selection between a natural and a cryptic 5' splice site.

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