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首页> 外文期刊>Molecular and Cellular Biology >Aspergillus nidulans wetA activates spore-specific gene expression.
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Aspergillus nidulans wetA activates spore-specific gene expression.

机译：构巢曲霉wetA激活孢子特异性基因表达。

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The Aspergillus nidulans wetA gene is required for synthesis of cell wall layers that make asexual spores (conidia) impermeable. In wetA mutant strains, conidia take up water and autolyze rather than undergoing the final stages of maturation. wetA is activated during conidiogenesis by sequential expression of the brlA and abaA regulatory genes. To determine whether wetA regulates expression of other sporulation-specific genes, its coding region was fused to a nutritionally regulated promoter that permits gene activation in vegetative cells (hyphae) under conditions that suppress conidiation. Expression of wetA in hyphae inhibited growth and caused excessive branching. It did not lead to activation of brlA or abaA but did cause accumulation of transcripts from genes that are normally expressed specifically during the late stages of conidiation and whose mRNAs are stored in mature spores. Thus, wetA directly or indirectly regulates expression of some spore-specific genes. At least one gene (wA), whose mRNA does not occur in spores but rather accumulates in the sporogenous phialide cells, was activated by wetA, suggesting that wetA may have a regulatory function in these cells as well as in spores. We propose that wetA is responsible for activating a set of genes whose products make up the final two conidial wall layers or direct their assembly and through this activity is responsible for acquisition of spore dormancy.
机译：构巢曲霉wetA基因是使无性孢子（分生孢子）不可渗透的细胞壁层合成所必需的。在wetA突变菌株中，分生孢子吸收水分并自溶，而不是经历成熟的最后阶段。通过连续表达brlA和abaA调控基因，在分生过程中激活wetA。为了确定wetA是否调节其他孢子特异性基因的表达，将其编码区与营养调节的启动子融合，该启动子可在抑制分裂的条件下在营养细胞（菌丝）中激活基因。菌丝中wetA的表达抑制生长并引起过度分支。它不会导致brlA或abaA的激活，但会导致转录的积累，这些转录本通常在分生后期特别表达，并且其mRNA储存在成熟的孢子中。因此，wetA直接或间接调节某些孢子特异性基因的表达。至少一个基因（wA）的mRNA不出现在孢子中，而是积累在孢子状的phialide细胞中，它被wetA激活，表明wetA在这些细胞以及孢子中都可能具有调节功能。我们建议wetA负责激活一组基因，这些基因的产物组成最后的两个分生孢子壁层或指导它们的组装，并通过此活动负责获得孢子休眠。

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