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首页> 外文期刊>Molecular and Cellular Biology >A muscle-specific intron enhancer required for rescue of indirect flight muscle and jump muscle function regulates Drosophila tropomyosin I gene expression.
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A muscle-specific intron enhancer required for rescue of indirect flight muscle and jump muscle function regulates Drosophila tropomyosin I gene expression.

机译：抢救间接飞行的肌肉和跳跃肌肉功能所需的特定于肌肉的内含子增强子可调节果蝇原肌球蛋白I基因的表达。

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摘要

The control of expression of the Drosophila melanogaster tropomyosin I (TmI) gene has been investigated by P-element transformation and rescue of the flightless and jumpless TmI mutant strain, Ifm(3)3. To localize cis-acting DNA sequences that control TmI gene expression, Ifm(3)3 flies were transformed with P-element plasmids containing various deletions and rearrangements of the TmI gene. The effects of these mutations on TmI gene expression were studied by analyzing both the extent of rescue of the Ifm(3)3 mutant phenotypes and determining TmI RNA levels in the transformed flies by primer extension analysis. The results of our analysis indicate that a region located within intron 1 of the gene is necessary and sufficient for directing muscle-specific TmI expression in the adult fly. This intron region has characteristics of a muscle regulatory enhancer element that can function in conjunction with the heterologous nonmuscle hsp70 promoter to promote rescue of the mutant phenotypes and to direct expression of an hsp70-Escherichia coli lacZ reporter gene in adult muscle. The enhancer can be subdivided further into two domains of activity based on primer extension analysis of TmI mRNA levels and on the rescue of mutant phenotypes. One of the intron domains is required for expression in the indirect flight muscle of the adult. The function of the second domain is unknown, but it could regulate the level of expression or be required for expression in other muscle.
机译：果蝇果蝇原肌球蛋白I（TmI）基因的表达控制已通过P元素转化和飞行的和跳跃的TmI突变株Ifm（3）3进行了研究。为了定位控制TmI基因表达的顺式作用DNA序列，Ifm（3）3果蝇用含有TmI基因的各种缺失和重排的P元素质粒转化。通过分析Ifm（3）3突变表型的拯救程度和通过引物延伸分析确定转化果蝇中的TmI RNA水平，研究了这些突变对TmI基因表达的影响。我们的分析结果表明，位于基因内含子1内的区域对于指导成年果蝇中肌肉特异性TmI表达是必要和充分的。该内含子区域具有肌肉调节增强子元件的特征，其可以与异源非肌肉hsp70启动子一起发挥功能，以促进突变表型的抢救并指导hsp70-大肠杆菌lacZ报告基因在成年肌肉中的表达。基于TmI mRNA水平的引物延伸分析和突变表型的拯救，增强子可进一步细分为两个活性域。在成人的间接飞行肌肉中表达需要内含子结构域之一。第二结构域的功能是未知的，但是它可以调节表达水平或在其他肌肉中表达所必需。

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