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首页> 外文期刊>Molecular and Cellular Biology >Proviral rearrangements and overexpression of a new cellular gene (nov) in myeloblastosis-associated virus type 1-induced nephroblastomas.
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Proviral rearrangements and overexpression of a new cellular gene (nov) in myeloblastosis-associated virus type 1-induced nephroblastomas.

机译：前病毒重排和新的细胞基因（nov）在成纤维细胞相关的1型病毒肾母细胞瘤中的表达。

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Histological and anatomopathological studies performed on 152 independent myeloblastosis-associated virus type 1 (MAV1)-induced nephroblastomas allowed us to precisely define the chronology of tumor development in chickens. Three tumors representing increasing developmental stages were used to construct genomic libraries and to study both the state of proviral genomes and the sites of MAV1 integration in genomic DNA. We established that increasing levels of proviral rearrangement, eventually leading to the elimination of infectious MAV genomes, were associated with tumor progression and that 22 individual tumors, representative of different developmental stages, did not contain any common MAV1 integration site. Cloning of cellular fragments flanking the MAV1-related proviruses in tumor DNA showed that each one of eight nephroblastomas tested expressed a high level of an as yet unidentified cellular gene (nov) whose transcription is normally arrested in adult kidney cells. Cloning of the normal nov gene established that in one tumor, fused long terminal repeat-truncated nov mRNA species were expressed, indicating that at least in that case, the high level of nov expression was under the control of the MAV long terminal repeat promoter. The normal nov gene encodes a putative 32-kDa secreted polypeptide, which is a member of a new family of proteins likely to be involved in cell growth regulation. We also showed that the expression of an amino-terminal-truncated nov product in chicken embryo fibroblasts was sufficient to induce their transformation.
机译：对152个独立的成纤维细胞病相关病毒1型（MAV1）诱导的肾母细胞瘤进行的组织学和解剖病理学研究使我们能够精确地确定鸡的肿瘤发展时间。使用三种代表发育阶段增加的肿瘤来构建基因组文库，并研究前病毒基因组的状态以及基因组DNA中MAV1整合位点。我们确定增加水平的前病毒重排，最终导致消除感染性MAV基因组，与肿瘤进展有关，并且代表22个个体肿瘤（代表不同的发育阶段）不包含任何常见的MAV1整合位点。克隆肿瘤DNA中与MAV1相关的前病毒两侧的细胞片段表明，测试的八种肾母细胞瘤中的每一种均表达高水平的尚未鉴定的细胞基因（nov），其转录通常在成年肾细胞中被阻滞。正常nov基因的克隆确定在一种肿瘤中表达融合的长末端重复截短的nov mRNA种类，这表明至少在这种情况下，nov表达的高水平在MAV长末端重复启动子的控制下。正常的nov基因编码一个推测的32 kDa分泌多肽，该多肽是可能参与细胞生长调节的新蛋白质家族的成员。我们还表明，在鸡胚成纤维细胞中氨基末端截短的nov产物的表达足以诱导其转化。

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