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首页> 外文期刊>Molecular and Cellular Biology >Raf-1 protein kinase is an integral component of the oncogenic signal cascade shared by epidermal growth factor and platelet-derived growth factor.
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Raf-1 protein kinase is an integral component of the oncogenic signal cascade shared by epidermal growth factor and platelet-derived growth factor.

机译：Raf-1蛋白激酶是表皮生长因子和血小板衍生生长因子共有的致癌信号级联的组成部分。

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摘要

Our recent studies with cell mutants indicate that a cascade shared by the epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) signals exists in NRK cells and mediates oncogenic signals induced by many oncogenes (A. Masuda, S. Kizaka-Kondoh, H. Miwatani, Y. Terada, H. Nojima, and H. Okayama, New Biol. 4:489-503, 1992). We have employed the antisense RNA technique to investigate possible involvement of Raf-1 kinase in this signal transduction cascade. NRK cell clones highly reduced in the Raf-1 production are generated by the expression of a c-raf-1 antisense RNA. They have no apparent growth defects and retain proper mitotic responses to growth factors but are refractory to transformation by EGF or PDGF plus transforming growth factor beta, v-erbB, v-fms, v-K-ras, v-mos, v-fos, v-src, simian virus 40 large T, and polyomavirus middle T but not by v-raf or adenovirus E1A. These results not only support our model for the oncogenic signal cascade but also lead to the conclusion that Raf-1 protein kinase is a downstream component of this oncogenic signal cascade shared by EGF and PDGF.
机译：我们最近对细胞突变体的研究表明，NRK细胞中存在表皮生长因子（EGF）和血小板衍生生长因子（PDGF）信号共有的级联，并介导许多致癌基因诱导的致癌信号（A. Masuda，S。Kizaka- Kondoh，H.Miwatani，Y.Terada，H.Nojima和H.Okayama，New Biol.4：489-503，1992）。我们已使用反义RNA技术来研究Raf-1激酶可能参与此信号转导级联反应。通过表达c-raf-1反义RNA产生了Raf-1产量大大降低的NRK细胞克隆。它们没有明显的生长缺陷，并且对生长因子保持适当的有丝分裂反应，但是对通过EGF或PDGF加上转化生长因子beta，v-erbB，v-fms，vK-ras，v-mos，v-fos，v的转化是难治的-src，猿猴病毒40大T和多瘤病毒中T，但不通过v-raf或腺病毒E1A进行。这些结果不仅支持我们的致癌信号级联模型，而且得出结论：Raf-1蛋白激酶是EGF和PDGF共享的该致癌信号级联的下游成分。

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