An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells.

B A Hosler; M B Rogers; C A Kozak; L J Gudas

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An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells.

机译：八聚体基序有助于在F9畸胎癌细胞中表达视黄酸调节的锌指基因Rex-1（Zfp-42）。

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The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for the Rex-1 gene (Zfp-42), characterized the gene's structure, and mapped the gene to mouse chromosome 8. Promoter elements contributing to the regulation of the Rex-1 promoter in F9 cells have been identified. A region required for Rex-1 promoter activity in F9 stem cells contains an octamer motif (ATTTGCAT) which is a binding site for octamer transcription factor members of the POU domain family of DNA-binding proteins. Rex-1 reporter plasmids including this octamer site also exhibited reduced expression in F9 cells treated with RA. Thus, the octamer motif is a regulatory element required for the activity of the Rex-1 promoter in F9 stem cells, and this motif contributes to the negative regulation by RA of the transcription of the Rex-1 gene. As an initial confirmation of the in vivo relevance of the isolated fragment, a larger Rex-1 promoter fragment, also containing the octamer site, was able to promote expression of the bacterial lacZ gene in mouse embryos at the morula stage.

机译：锌指基因Rex-1（Zfp-42）的信息在未分化的小鼠F9畸胎瘤细胞和胚胎干细胞中表达。当通过添加视黄酸（RA）进行分化诱导F9细胞时，Rex-1的表达在转录水平上会降低。我们已经分离出Rex-1基因（Zfp-42）的基因组DNA，表征了该基因的结构，并将该基因定位于小鼠8号染色体。已确定了在F9细胞中调控Rex-1启动子的启动子。 F9干细胞中Rex-1启动子活性所需的区域包含一个八聚体基序（ATTTGCAT），它是DNA结合蛋白POU域家族的八聚体转录因子成员的结合位点。包含该八聚体位点的Rex-1报告基因质粒在用RA处理的F9细胞中也表现出降低的表达。因此，八聚体基序是F9干细胞中Rex-1启动子活性所需的调节元件，并且该基序有助于RA对Rex-1基因的转录进行负调控。作为分离片段的体内相关性的初步确认，更大的Rex-1启动子片段（也包含八聚体位点）能够在桑ula期促进小鼠lacZ基因在小鼠胚胎中的表达。

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《Molecular and Cellular Biology》 |1993年第5期|共10页
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B A Hosler; M B Rogers; C A Kozak; L J Gudas;
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中图分类细胞生物学;
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1. Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells. [J] . B A Hosler, G J LaRosa, J F Grippo, Molecular and Cellular Biology . 1989,第12期

机译：维甲酸在F9畸胎癌细胞中会迅速降低REX-1（一种含锌指基序的基因）的表达。
2. Retinoic acid induces parietal endoderm but not primitive endoderm and visceral endoderm differentiation in F9 teratocarcinoma stem cells with a targeted deletion of the Rex-1 (Zfp-42) gene. [J] . Thompson J, Gudas L Molecular and Cellular Endocrinology . 2002,第1a2期

机译：维甲酸可诱导F9畸胎癌干细胞的顶叶内胚层分化，但不诱导原始内胚层和内脏内胚层分化，并有针对性地缺失Rex-1（Zfp-42）基因。
3. Overexpression of the cellular retinoic acid binding protein-I (CRABP-I) results in a reduction in differentiation-specific gene expression in F9 teratocarcinoma cells. [J] . J F Boylan, L J Gudas Journal of cell biology . 1991,第5期

机译：细胞视黄酸结合蛋白-I（CRABP-1）的过表达导致F9畸胎癌细胞中分化特异性基因表达的减少。
4. The Duplication and Intragenic Domain Expansion of Human C2H2 Zinc Finger Genes Are Associated with Transposable Elements and Relevant to the Expression-based Clustering [C] . Wensheng Zhang, Andrea Edwards, Prescott Deininger, International Conference on Bioinformatics and Computational Biology . 2015

机译：人C2H2锌指基因的重复和腺体结构域膨胀与转换元件相关并与基于表达的聚类相关联
5. Characterization of the zinc finger gene Rex-1 (Zfp-42) whose expression is reduced in murine F9 embryonal carcinoma cells treated with retinoic acid. [D] . Hosler, Betsy Ann. 1993

机译：锌指基因Rex-1（Zfp-42）的表征，在用视黄酸处理的小鼠F9胚胎癌细胞中其表达降低。
6. An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells. [O] . B A Hosler, M B Rogers, C A Kozak, 1993

机译：八聚体基序有助于在F9畸胎癌细胞中表达视黄酸调节的锌指基因Rex-1（Zfp-42）。
7. An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells. [O] . Hosler, B A, Rogers, M B, Kozak, C A, 1993

机译：八聚体基序有助于在F9畸胎癌细胞中表达视黄酸调节的锌指基因Rex-1（Zfp-42）。
8. Metal Occupancy of Zinc Finger Motifs as Determinants for Zn2+-Mediated Chemosensitization of Prostate Cancer Cells. [R] . W. H. Gmeiner 2013

机译：锌指基序的金属占据作为Zn2 +介导的前列腺癌细胞化学增敏的决定因素。

An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells.

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