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首页> 外文期刊>Molecular and Cellular Biology >CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c.
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CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c.

机译：CYC2编码参与酵母细胞色素c的线粒体导入的因子。

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The gene CYC2 from the yeast Saccharomyces cerevisiae was previously shown to affect levels of mitochondrial cytochrome c by acting at a posttranslational step in cytochrome c biosynthesis. We report here the cloning and identification of the CYC2 gene product as a protein involved in import of cytochrome c into mitochondria. CYC2 encodes a 168-amino-acid open reading frame with at least two potential transmembrane segments. Antibodies against a synthetic peptide corresponding to the carboxyl terminus of the predicted sequence were raised. These antibodies recognize multiple bands on immunoblots of mitochondrial extracts. The intensities of these bands vary according to the gene dosage of CYC2 in various isogenic strains. Immunoblotting of subcellular fractions suggests that the CYC2 gene product is a mitochondrial protein. Deletion of CYC2 leads to accumulation of apocytochrome c in the cytoplasm. However, strains with deletions of this gene still import low levels of cytochrome c into mitochondria. The effects of cyc2 mutations are more pronounced in rho- strains than in rho+ strains, even though rho- strains that are CYC2+ contain normal levels of holocytochrome c. cyc2 mutations affect levels of iso-1-cytochrome c more than they do levels of iso-2-cytochrome c, apparently because of the greater susceptibility of apo-iso-1-cytochrome c to degradation in the cytoplasm. We propose that CYC2 encodes a factor that increases the efficiency of cytochrome c import into mitochondria.
机译：先前显示来自酿酒酵母的基因CYC2通过在细胞色素c生物合成的翻译后步骤起作用来影响线粒体细胞色素c的水平。我们在这里报告了CYC2基因产物的克隆和鉴定，该产物是涉及细胞色素c进入线粒体进口的蛋白质。 CYC2编码具有至少两个潜在跨膜片段的168个氨基酸的开放阅读框。产生了针对对应于预测序列的羧基末端的合成肽的抗体。这些抗体识别线粒体提取物免疫印迹上的多个条带。这些条带的强度根据各种同基因菌株中CYC2的基因剂量而变化。亚细胞部分的免疫印迹表明CYC2基因产物是一种线粒体蛋白。 CYC2的缺失导致脱细胞色素c在细胞质中的积累。但是，具有该基因缺失的菌株仍将低水平的细胞色素c导入线粒体。 CYC2突变的影响在rho-菌株中比在rho +菌株中更为明显，即使CYC2 +的rho-菌株含有正常水平的全细胞色素c。 cyc2突变对iso-1-细胞色素c的影响比对iso-2-细胞色素c的影响更大，这显然是因为apo-iso-1-cytochrome c对细胞质降解的敏感性更高。我们建议CYC2编码增加细胞色素c导入线粒体效率的因子。

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