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首页> 外文期刊>Molecular and Cellular Biology >Identification and characterization of a beta-globin promoter-binding factor from murine erythroleukemia cells.
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Identification and characterization of a beta-globin promoter-binding factor from murine erythroleukemia cells.

机译：鉴定和鉴定鼠红白血病细胞中β-珠蛋白启动子结合因子。

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摘要

We have identified a DNA-binding activity with specificity for the beta DRE, an evolutionarily conserved transcriptional regulatory element in mammalian adult beta-globin promoters. This binding activity, which we term beta DRf, for beta-globin direct repeat factor, was detected in fractionated nuclear extracts from the murine erythroleukemia cell line and has been partially purified from undifferentiated cells. beta DRf makes symmetric contacts on the two copies of its recognition sequence on both strands and introduces a bend into the DNA helix upon binding. While the factor displays a low binding affinity for the beta DRE in isolation, it binds to the intact beta-globin promoter and DNA fragments containing multiple beta DRE-binding sites with high affinity. A correlation between beta DRf binding affinity and transcriptional activity of beta DRE mutant promoters suggests that this factor stimulates transcription of the beta-globin promoter in vivo.
机译：我们已经确定了对βDRE具有特异性的DNA结合活性，βDRE是哺乳动物成年β-珠蛋白启动子中进化上保守的转录调控元件。这种结合活性，我们称其为β-珠蛋白直接重复因子的βDRf，是在鼠红白血病细胞系的分级核提取物中检测到的，并已从未分化的细胞中部分纯化。 βDRf在两条链上的其识别序列的两个副本上进行对称接触，并在结合后将弯曲引入DNA螺旋中。尽管该因子单独显示出对βDRE的低结合亲和力，但它以高亲和力结合完整的β-珠蛋白启动子和包含多个βDRE结合位点的DNA片段。 βDRf结合亲和力与βDRE突变启动子的转录活性之间的相关性表明该因子在体内刺激了β珠蛋白启动子的转录。

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