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首页> 外文期刊>Molecular and Cellular Biology >Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer.
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Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer.

机译：重叠的正调节元件和负调节元件确定δ1-晶状蛋白增强子的晶状体特异性活性。

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摘要

Lens-specific expression of the delta 1-crystallin gene is governed by an enhancer in the third intron, and the 30-bp-long DC5 fragment was found to be responsible for eliciting the lens-specific activity. Mutational analysis of the DC5 fragment identified two contiguous, interdependent positive elements and a negative element which overlaps the 3'-located positive element. Previously identified ubiquitous factors delta EF1 bound to the negative element and repressed the enhancer activity in nonlens cells. Mutation and cotransfection analyses indicated the existence of an activator which counteracts the action of delta EF1 in lens cells, probably through binding site competition. We also found a group of nuclear factors, collectively called delta EF2, which bound to the 5'-located positive element. delta EF2a and -b were the major species in lens cells, whereas delta EF2c and -d predominated in nonlens cells. These delta EF2 proteins probably cooperate with factors bound to the 3'-located element in activation in lens cells and repression in nonlens cells. delta EF2 proteins also bound to a promoter sequence of the gamma F-crystallin gene, suggesting that delta EF2 proteins are involved in lens-specific regulation of various crystallin classes.
机译：δ1-crystallin基因的晶状体特异性表达由第三内含子中的增强子控制，并且发现30 bp长的DC5片段负责引起晶状体特异性活性。 DC5片段的突变分析确定了两个连续的，相互依存的正元素和一个与3'位正元素重叠的负元素。先前确定的普遍存在的因素三角洲EF1绑定到负元素，并抑制非透镜细胞中的增强子活性。突变和共转染分析表明，可能通过结合位点竞争抵消了晶状体细胞中δEF1的作用。我们还发现了一组核因子，统称为δEF2，与5'阳性元素结合。 δEF2a和-b是晶状体细胞中的主要物种，而EF2c和-dδ在非晶状体细胞中占主导。这些δEF2蛋白可能与晶状体细胞激活和非晶状体细胞抑制中与3'定位元件结合的因子协同作用。 δEF2蛋白也与γF-晶状体蛋白基因的启动子序列结合，这表明δEF2蛋白参与了各种晶状蛋白类的晶状体特异性调控。

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