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首页> 外文期刊>Molecular and Cellular Biology >TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function.
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TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function.

机译：TIF4631和TIF4632：编码帽结合蛋白复合物（真核生物起始因子4F）的高分子量亚基的两个酵母基因包含RNA识别基序样序列，并发挥基本功能。

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The 5' ends of eukaryotic mRNAs are blocked by a cap structure, m7GpppX (where X is any nucleotide). The interaction of the cap structure with a cap-binding protein complex is required for efficient ribosome binding to the mRNA. In Saccharomyces cerevisiae, the cap-binding protein complex is a heterodimer composed of two subunits with molecular masses of 24 (eIF-4E, CDC33) and 150 (p150) kDa. p150 is presumed to be the yeast homolog of the p220 component of mammalian eIF-4F. In this report, we describe the isolation of yeast gene TIF4631, which encodes p150, and a closely related gene, TIF4632. TIF4631 and TIF4632 are 53% identical overall and 80% identical over a 320-amino-acid stretch in their carboxy-terminal halves. Both proteins contain sequences resembling the RNA recognition motif and auxiliary domains that are characteristic of a large family of RNA-binding proteins. tif4631-disrupted strains exhibited a slow-growth, cold-sensitive phenotype, while disruption of TIF4632 failed to show any phenotype under the conditions assayed. Double gene disruption engendered lethality, suggesting that the two genes are functionally homologous and demonstrating that at least one of them is essential for viability. These data are consistent with a critical role for the high-molecular-weight subunit of putative yeast eIF-4F in translation. Sequence comparison of TIF4631, TIF4632, and the human eIF-4F p220 subunit revealed significant stretches of homology. We have thus cloned two yeast homologs of mammalian p220.
机译：真核mRNA的5'末端被帽结构m7GpppX（其中X是任何核苷酸）封闭。帽结构与帽结合蛋白复合物的相互作用对于有效的核糖体与mRNA的结合是必需的。在酿酒酵母中，帽结合蛋白复合物是由两个亚基组成的异二聚体，分子量为24（eIF-4E，CDC33）和150（p150）kDa。推测p150是哺乳动物eIF-4F的p220组分的酵母同源物。在此报告中，我们描述了编码p150的酵母基因TIF4631和一个密切相关的基因TIF4632的分离。 TIF4631和TIF4632在其羧基末端两半的320个氨基酸序列中，整体具有53％的同一性，与80％的同一性。两种蛋白质均包含类似于RNA识别基序的序列和具有大量RNA结合蛋白家族特征的辅助结构域。破坏tif4631的菌株表现出缓慢生长，对冷敏感的表型，而在所分析的条件下，破坏TIF4632未能显示任何表型。双重基因破坏导致致死性，表明这两个基因在功能上是同源的，这表明它们中至少有一个对生存力至关重要。这些数据与推定酵母eIF-4F的高分子量亚基在翻译中的关键作用相一致。 TIF4631，TIF4632和人eIF-4F p220亚基的序列比较显示出明显的同源性。因此，我们已经克隆了哺乳动物p220的两个酵母同源物。

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