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首页> 外文期刊>Molecular and Cellular Biology >ADH2 expression is repressed by REG1 independently of mutations that alter the phosphorylation of the yeast transcription factor ADR1.
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ADH2 expression is repressed by REG1 independently of mutations that alter the phosphorylation of the yeast transcription factor ADR1.

机译：ADH2的表达受REG1的抑制，而与改变酵母转录因子ADR1磷酸化的突变无关。

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In Saccharomyces cerevisiae, expression of the ADH2 gene is undetectable during growth on glucose. The transcription factor ADR1 is required to fully activate expression when glucose becomes depleted. Partial activation during growth on glucose occurred in cells carrying a constitutive allele of ADR1 in which the phosphorylatable serine of a cyclic AMP (cAMP)-dependent protein kinase phosphorylation site had been changed to alanine. When glucose was removed from the growth medium, a substantial increase in the level of this constitutive expression was observed for both the ADH2 gene and a reporter construct containing the ADR1 binding site. This suggests that glucose can block ADR1-mediated activation independently of cAMP-dependent phosphorylation at serine 230. REG1/HEX2/SRN1 was identified as a potential serine 230-independent repressor of ADH2 expression. Yeast strains carrying a deletion of the REG1 gene, reg1-1966, showed a large increase in ADR1-dependent expression of ADH2 during growth on glucose. A smaller increase in ADR1-independent expression was also observed. Additionally, an increase in the level of ADR1 expression and posttranslational modification of the ADR1 protein were observed. When the reg1-1966 allele was combined with various ADR1 constitutive alleles, the level of ADH2 expression was synergistically elevated. This indicates that REG1 can act independently of phosphorylation at serine 230. Our results suggest that glucose repression in the presence of ADR1 constitutive alleles occurs primarily through a REG1-dependent pathway which appears to affect ADH2 transcription at multiple levels.
机译：在酿酒酵母中，在葡萄糖上生长期间无法检测到ADH2基因的表达。葡萄糖耗尽时，转录因子ADR1需要完全激活表达。在带有葡萄糖的生长过程中，部分激活发生在携带ADR1组成性等位基因的细胞中，其中环状AMP（cAMP）依赖性蛋白激酶磷酸化位点的可磷酸化丝氨酸已更改为丙氨酸。当从生长培养基中除去葡萄糖时，对于ADH2基因和含有ADR1结合位点的报道基因构建体，都观察到该组成型表达水平的显着增加。这表明葡萄糖可以独立于丝氨酸230上依赖cAMP的磷酸化而阻断ADR1介导的活化。REG1/ HEX2 / SRN1被鉴定为ADH2表达的潜在丝氨酸230依赖性阻遏物。携带REG1基因reg1-1966缺失的酵母菌株在葡萄糖生长期间显示ADH2的ADR1依赖性表达大幅增加。还观察到较小的ADR1独立表达增加。此外，观察到ADR1表达水平的提高和ADR1蛋白的翻译后修饰。当reg1-1966等位基因与各种ADR1组成型等位基因结合时，ADH2表达水平协同升高。这表明REG1可以独立于丝氨酸230的磷酸化起作用。我们的结果表明，在存在ADR1组成性等位基因的情况下，葡萄糖抑制主要通过REG1依赖性途径发生，该途径似乎在多个水平上影响ADH2转录。

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