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首页> 外文期刊>Molecular and Cellular Biology >Two distinct factors bind to the rabbit uteroglobin TATA-box region and are required for efficient transcription.
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Two distinct factors bind to the rabbit uteroglobin TATA-box region and are required for efficient transcription.

机译：两个不同的因子与兔子宫珠蛋白TATA盒区域结合，是有效转录所必需的。

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The rabbit uteroglobin gene is expressed in a variety of epithelial cell types like the lung Clara cells and the glandular and luminal epithelial cells of the endometrium. Expression in Clara cells is on a high constitutive level, whereas expression in the rabbit endometrium is under tight hormonal control. One important element of the rabbit uteroglobin gene mediating its efficient transcription in two epithelial cell lines from human endometrium (Ishikawa) and lung (NCI-H441) is its noncanonical TATA box (TACA). Here, we show that two factors (TATA core factor [TCF] and TATA palindrome factor [TPF]) different from the TATA-box binding protein bind to the DNA major groove at two adjacent sites within the uteroglobin TATA-box region and that one of them (TCF) is specifically expressed in cell lines derived from uteroglobin-expressing tissues. The binding sites for TCF and TPF, respectively, are both required for efficient transcription in Ishikawa and NCI-H441 cells. Mutation of the TACA box, which we show is a poor TATA box in functional terms, to a canonical TATA motif does not affect TCF and TPF binding. Therefore, we suggest that the function of the unusual cytosine could be to reduce rabbit uteroglobin expression in cells lacking TCF and that the interaction of TATA-box binding protein with the weak TACA site is facilitated in TCF- and TPF-positive cells.
机译：兔子宫珠蛋白基因在多种上皮细胞类型中表达，例如肺克拉拉细胞以及子宫内膜的腺体和管腔上皮细胞。 Clara细胞中的表达水平很高，而兔子宫内膜中的表达受到严格的激素控制。兔子宫珠蛋白基因在人子宫内膜（石川县）和肺部（NCI-H441）的两种上皮细胞系中介导其有效转录的一个重要元素是其非经典TATA盒（TACA）。在这里，我们显示与TATA-box结合蛋白不同的两个因子（TATA核心因子[TCF]和TATA回文因子[TPF]）结合到子宫珠蛋白TATA-box区域内两个相邻位点的DNA主沟上，它们（TCF）在源自表达子宫球蛋白的组织的细胞系中特异性表达。在石川和NCI-H441细胞中有效转录分别需要TCF和TPF的结合位点。从功能上来讲，我们展示的TACA盒向TATA母题的功能转变很差，它不会影响TCF和TPF的结合。因此，我们认为异常胞嘧啶的功能可能是减少缺乏TCF的细胞中兔子宫珠蛋白的表达，并且TCF-和TPF阳性细胞促进TATA-box结合蛋白与弱TACA位点的相互作用。

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