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首页> 外文期刊>Molecular and Cellular Biology >Definition of a lipopolysaccharide-responsive element in the 5'-flanking regions of MuRantes and crg-2.
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Definition of a lipopolysaccharide-responsive element in the 5'-flanking regions of MuRantes and crg-2.

机译：MuRantes和crg-2的5'侧翼区域中脂多糖响应元件的定义。

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Macrophages are stimulated by lipopolysaccharide (LPS) of gram-negative organisms. The changes in LPS-stimulated macrophages include transcriptional activation of multiple immediate-early genes, which may contribute to the natural immunity to microorganisms. We have defined by deletion and mutational analysis LPS-responsive elements (LREs) in two chemokine genes, MuRantes and crg-2, which are activated in an immediate-early manner. LRE consists of two motifs, TCAYR, which is an AP-1 half site with two flanking bases, and (A/T) (G/C)NTTYC(A/T)NTTY, which resembles in part the interferon-stimulated responsive element (ISRE). The orientation of these two motifs relative to each other in MuRantes differed from that in crg-2. These two motifs are separated by 10 and 6 nonconsensus nucleotides in the MuRantes and crg-2 LREs, respectively. Stimulation of macrophage-like RAW 264.7 cells with alpha/beta interferon did not activate MuRantes, indicating that the ISRE-like motif in MuRantes does not have ISRE activity. Upon stimulation of RAW 264.7 cells with LPS, proteins capable of binding to LRE accumulate in the nuclei as measured by electrophoretic mobility shift assay. These LRE-binding proteins include c-Jun and CREB.
机译：革兰氏阴性生物的脂多糖（LPS）刺激巨噬细胞。 LPS刺激的巨噬细胞的变化包括多个立即早期基因的转录激活，这可能有助于对微生物的天然免疫。通过删除和突变分析，我们已经定义了两个趋化因子基因MuRantes和crg-2中的LPS响应元件（LRE），它们立即被激活。 LRE由两个基序组成：TCAYR（是带有两个侧翼碱基的AP-1半位点）和（A / T）（G / C）NTTYC（A / T）NTTY，其部分类似于干扰素刺激的反应元件（ISRE）。 MuRantes中这两个基序相对于彼此的方向与crg-2中的相对。这两个基序分别在MuRantes和crg-2 LRE中被10个和6个非共有核苷酸隔开。用α/β干扰素刺激巨噬细胞样RAW 264.7细胞不会激活MuRantes，表明MuRantes中的ISRE样基序没有ISRE活性。用LPS刺激RAW 264.7细胞后，通过电泳迁移率迁移测定法测量，能够与LRE结合的蛋白质会在细胞核中积聚。这些LRE结合蛋白包括c-Jun和CREB。

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