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首页> 外文期刊>Molecular and Cellular Biology >Continued functioning of the secretory pathway is essential for ribosome synthesis.
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Continued functioning of the secretory pathway is essential for ribosome synthesis.

机译：分泌途径的持续功能对于核糖体合成至关重要。

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To explore the regulatory elements that maintain the balanced synthesis of the components of the ribosome, we isolated a temperature-sensitive (ts) mutant of Saccharomyces cerevisiae in which transcription both of rRNA and of ribosomal protein genes is defective at the nonpermissive temperature. Temperature sensitivity for growth is recessive and segregates 2:2. A gene that complements the ts phenotype was cloned from a genomic DNA library. Sequence analysis revealed that this gene is SLY1, encoding a protein essential for protein and vesicle transport between the endoplasmic reticulum and the Golgi apparatus. In the strain carrying our ts allele of SLY1, accumulation of the carboxypeptidase Y precursor was detected at the nonpermissive temperature, indicating that the secretory pathway is defective. To ask whether the effect of the ts allele on ribosome synthesis was specific for sly1 or was a general result of the inactivation of the secretion pathway, we assayed the levels of mRNA for several ribosomal proteins in cells carrying ts alleles of sec1, sec7, sec11, sec14, sec18, sec53, or sec63, representing all stages of secretion. In each case, the mRNA levels were severely depressed, suggesting that this is a common feature in mutants of protein secretion. For the mutants tested, transcription of rRNA was also substantially reduced. Furthermore, treatment of a sensitive strain with brefeldin A at a concentration sufficient to block the secretion pathway also led to a decrease of the level of ribosomal protein mRNA, with kinetics suggesting that the effect of a secretion defect is manifest within 15 to 30 min. We conclude that the continued function of the entire secretion pathway is essential for the maintenance of ribosome synthesis. The apparent coupling of membrane synthesis and ribosome synthesis suggest the existence of a regulatory network that connects the production of the various structural elements of the cell.
机译：为了探索维持核糖体成分平衡合成的调控元件，我们分离了酿酒酵母的温度敏感（ts）突变体，其中在不允许的温度下rRNA和核糖体蛋白基因的转录均存在缺陷。生长的温度敏感性是隐性的，且以2：2隔离。从基因组DNA文库中克隆了与ts表型互补的基因。序列分析表明，该基因是SLY1，编码内质网和高尔基体之间的蛋白质和囊泡运输必需的蛋白质。在携带我们的SLY1 ts等位基因的菌株中，在非容许温度下检测到羧肽酶Y前体的积累，表明分泌途径有缺陷。为了问问ts等位基因对核糖体合成的作用是sly1特异还是分泌途径失活的一般结果，我们在携带sec1，sec7，sec11 ts等位基因的细胞中检测了几种核糖体蛋白的mRNA水平。 ，sec14，sec18，sec53或sec63，代表分泌的所有阶段。在每种情况下，mRNA水平都严重降低，表明这是蛋白分泌突变体的共同特征。对于测试的突变体，rRNA的转录也大大减少。此外，以足以阻断分泌途径的浓度的布雷菲德菌素A处理敏感菌株也导致核糖体蛋白mRNA水平的降低，动力学表明分泌缺陷的作用在15至30分钟内显现。我们得出结论，整个分泌途径的持续功能对于维持核糖体合成至关重要。膜合成与核糖体合成的表观耦合表明存在连接细胞各种结构元件生产的调节网络。

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