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首页> 外文期刊>Molecular and Cellular Biology >Repression of a herpes simplex virus immediate-early promoter by the Oct-2 transcription factor is dependent on an inhibitory region at the N terminus of the protein.
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Repression of a herpes simplex virus immediate-early promoter by the Oct-2 transcription factor is dependent on an inhibitory region at the N terminus of the protein.

机译：Oct-2转录因子对单纯疱疹病毒立即早期启动子的抑制取决于该蛋白N端的抑制区。

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摘要

The B-cell form of the Oct-2 transcription factor Oct 2.1 can activate the herpes simplex virus immediate-early gene 3 (IE3) promoter, whereas the neuronally expressed Oct 2.4 and 2.5 forms of the protein, which contain a different C terminus, can repress this promoter. Here we show that partial or full deletion of the C terminus of Oct 2.1 in the presence of an intact N terminus results in a protein which can strongly repress the IE3 promoter. In contrast, deletion of the entire N terminus or a short region within it leaving the C terminus intact results in a very strong activator. Deletion of both N and C termini leaving only the isolated POU domain generates only a very weak repressor. The N-terminal region defined in this way can repress a heterologous promoter when linked to the DNA-binding domain of the GAL4 factor, indicating that it can function as an independent inhibitory domain. These results indicate that a specific region within the N terminus common to Oct 2.1, 2.4, and 2.5 plays a critical role in the ability of neuronally expressed forms of Oct-2 to repress the IE3 promoter but can do so only when the C-terminal region of Oct 2.1 is altered or deleted.
机译：Oct-2转录因子Oct 2.1的B细胞形式可以激活单纯疱疹病毒即刻早期基因3（IE3）启动子，而神经元表达的蛋白Oct 2.4和2.5形式包含不同的C末端，可以抑制这个启动子。在这里，我们显示在完整的N末端存在下，Oct 2.1的C末端部分或全部缺失会导致一种蛋白质，该蛋白质可以强烈抑制IE3启动子。相反，整个N末端或其中一个短区域的缺失使C末端完整无缺，从而产生了非常强大的激活剂。 N和C末端的删除仅留下孤立的POU域仅产生非常弱的阻遏物。当以这种方式定义的N末端区域与GAL4因子的DNA结合结构域连接时，可以抑制异源启动子，表明其可以起独立的抑制域的作用。这些结果表明，Oct 2.1、2.4和2.5共有的N末端内的特定区域在神经元表达形式的Oct-2抑制IE3启动子的能力中起着关键作用，但只有在C末端时才能这样做10月2.1的区域被更改或删除。

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