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首页> 外文期刊>Molecular and Cellular Biology >The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex.
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The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex.

机译：酵母羧基末端重复结构域激酶CTDK-1是发散的细胞周期蛋白-细胞周期蛋白依赖性激酶复合物。

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摘要

Saccharomyces cerevisiae CTDK-I is a protein kinase complex that specifically and efficiently hyperphosphorylates the carboxyl-terminal repeat domain (CTD) of RNA polymerase II and is composed of three subunits of 58, 38, and 32 kDa. The kinase is essential in vivo for normal phosphorylation of the CTD and for normal growth and differentiation. We have now cloned the genes for the two smaller kinase subunits, CTK2 and CTK3, and found that they form a unique, divergent cyclin-cyclin-dependent kinase complex with the previously characterized largest subunit protein CTK1, a cyclin-dependent kinase homolog. The CTK2 gene encodes a cyclin-related protein with limited homology to cyclin C, while CTK3 shows no similarity to other known proteins. Copurification of the three gene products with each other and CTDK-I activity by means of conventional chromatography and antibody affinity columns has verified their participation in the complex in vitro. In addition, null mutations of each of the genes and all combinations thereof conferred very similar growth-impaired, cold-sensitive phenotypes, consistent with their involvement in the same function in vivo. These characterizations and the availability of all of the genes encoding CTDK-I and reagents derivable from them will facilitate investigations into CTD phosphorylation and its functional consequences both in vivo and in vitro.
机译：啤酒酵母CTDK-1是一种蛋白激酶复合物，其特异性和有效地使RNA聚合酶II的羧基末端重复结构域（CTD）磷酸化，并由58、38和32 kDa的三个亚基组成。该激酶在体内对于CTD的正常磷酸化以及正常的生长和分化必不可少。现在，我们已经克隆了两个较小的激酶亚基CTK2和CTK3的基因，发现它们与以前表征的最大亚基蛋白CTK1（细胞周期蛋白依赖性激酶同源物）形成了独特的，发散的细胞周期蛋白-细胞周期蛋白依赖性激酶复合物。 CTK2基因编码与细胞周期蛋白C同源性有限的细胞周期蛋白相关蛋白，而CTK3与其他已知蛋白没有相似性。通过常规色谱和抗体亲和柱对三种基因产物彼此共纯化以及CTDK-1活性已证明它们在体外参与了该复合物。另外，每个基因及其所有组合的无效突变赋予非常相似的生长受损的冷敏感表型，这与它们参与体内的相同功能一致。这些表征以及所有编码CTDK-1的基因以及从其衍生的试剂的可用性将有助于研究CTD磷酸化及其在体内和体外的功能后果。

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