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首页> 外文期刊>Molecular and Cellular Biology >A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus.
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A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus.

机译：核孔蛋白FG重复域在人类免疫缺陷病毒1型Rev蛋白和RNA从细胞核输出中的作用。

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摘要

The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae. The Rev NES has been shown to specifically interact with a human (hRIP/RAB1) and a yeast (yRip1p) protein in the two-hybrid assay. Both of these interacting proteins are related to FG nucleoporins on the basis of the presence of typical repeat motifs. This paper shows that Rev is able to interact with multiple FG repeat-containing nucleoporins from both S. cerevisiae and mammals; moreover, the ability of Rev NES mutants to interact with these FG nucleoporins parallels the ability of the mutants to promote RNA export in yeast and mammalian cells. The data also show that, after Xenopus oocyte nuclear injection, several FG nucleoporin repeat domains inhibit the export of both Rev protein and U small nuclear RNAs, suggesting that these nucleoporins participate in Rev-mediated and cellular RNA export. Interestingly, not all FG nucleoporin repeat domains produced the same pattern of RNA export inhibition. The results suggest that Rev and cellular mediators of RNA export can interact with multiple components of the nuclear pore complex during transport, analogous to the proposed mode of action of the nuclear protein import receptor.
机译：人类免疫缺陷病毒1型Rev蛋白包含一个核输出信号（NES），该信号是Rev介导的RNA在哺乳动物以及酵母酵母中输出所必需的。 Rev NES已显示在两种杂交试验中与人（hRIP / RAB1）和酵母（yRip1p）蛋白特异性相互作用。基于典型重复基序的存在，这两个相互作用的蛋白均与FG核孔蛋白有关。本文显示，Rev能够与酿酒酵母和哺乳动物的多个含有FG重复序列的核孔蛋白相互作用。此外，Rev NES突变体与这些FG核孔蛋白相互作用的能力与该突变体在酵母和哺乳动物细胞中促进RNA输出的能力平行。数据还表明，在非洲爪蟾卵母细胞核注射后，几个FG核孔蛋白重复域抑制了Rev蛋白和U小核RNA的输出，表明这些核孔蛋白参与了Rev介导的和细胞RNA的输出。有趣的是，并非所有的FG核孔蛋白重复域都产生相同的RNA输出抑制模式。结果表明，RNA转运的Rev和细胞介体可以在运输过程中与核孔复合体的多个成分相互作用，类似于拟议的核蛋白输入受体作用方式。

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