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首页> 外文期刊>Molecular and Cellular Biology >Characterization of elk, a brain-specific receptor tyrosine kinase.
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Characterization of elk, a brain-specific receptor tyrosine kinase.

机译：麋鹿的特征，一种大脑特异性受体酪氨酸激酶。

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The elk gene encodes a novel receptorlike protein-tyrosine kinase, which belongs to the eph subfamily. We have previously identified a partial cDNA encompassing the elk catalytic domain (K. Letwin, S.-P. Yee, and T. Pawson, Oncogene 3:621-678, 1988). Using this cDNA as a probe, we have isolated cDNAs spanning the entire rat elk coding sequence. The predicted Elk protein contains all the hallmarks of a receptor tyrosine kinase, including an N-terminal signal sequence, a cysteine-rich extracellular domain, a membrane-spanning segment, a cytoplasmic tyrosine kinase domain, and a C-terminal tail. In both amino acid sequence and overall structure, Elk is most similar to the Eph and Eck protein-tyrosine kinases, suggesting that the eph, elk, and eck genes encode members of a new subfamily of receptorlike tyrosine kinases. Among rat tissues, elk expression appears restricted to brain and testes, with the brain having higher levels of both elk RNA and protein. Elk protein immunoprecipitated from a rat brain lysate becomes phosphorylated on tyrosine in an in vitro kinase reaction, consistent with the prediction that the mammalian elk gene encodes a tyrosine kinase capable of autophosphorylation. The characteristics of the Elk tyrosine kinase suggest that it may be involved in cell-cell interactions in the nervous system.
机译：麋鹿基因编码一种新的受体样蛋白酪氨酸激酶，属于eph亚家族。我们先前已经鉴定了包含麋鹿催化结构域的部分cDNA（K.Letwin，S.-P.Yee，和T.Pawson，Oncogene 3：621-678，1988）。使用该cDNA作为探针，我们分离了跨越整个大鼠麋鹿编码序列的cDNA。预测的麋鹿蛋白包含受体酪氨酸激酶的所有标志，包括N端信号序列，富含半胱氨酸的胞外域，跨膜区段，胞质酪氨酸激酶域和C末端尾巴。在氨基酸序列和总体结构上，Elk与Eph和Eck蛋白酪氨酸激酶最相似，这表明eph，elk和eck基因编码受体样酪氨酸激酶新亚家族的成员。在大鼠组织中，麋鹿的表达似乎仅限于大脑和睾丸，大脑的麋鹿RNA和蛋白质水平较高。从大鼠脑溶解物中免疫沉淀的麋鹿蛋白在体外激酶反应中在酪氨酸上被磷酸化，这与哺乳动物麋鹿基因编码能够自磷酸化的酪氨酸激酶的预测相符。 Elk酪氨酸激酶的特征表明它可能参与神经系统中的细胞间相互作用。

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