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首页> 外文期刊>Molecular and Cellular Biology >Substitutions in the hydrophobic core of the alpha-factor receptor of Saccharomyces cerevisiae permit response to Saccharomyces kluyveri alpha-factor and to antagonist.
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Substitutions in the hydrophobic core of the alpha-factor receptor of Saccharomyces cerevisiae permit response to Saccharomyces kluyveri alpha-factor and to antagonist.

机译：酿酒酵母α因子受体的疏水核心中的取代允许对克鲁维酵母酵母α因子和拮抗剂的反应。

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摘要

Mutations in the Saccharomyces cerevisiae alpha-factor receptor that lead to improved response to Saccharomyces kluyveri alpha-factor were identified and sequenced. Mutants were isolated from cells bearing randomly mutagenized receptor gene (STE2) plasmids by an in vivo screen. Five mutations lead to substitutions in hydrophobic segments in the core of the receptor (M54I, S145L, S145L-S219L, A229V, L255S-S288P). Remarkably, strains expressing these mutant receptors exhibited positive pheromone responses to desTrp1,Ala3-alpha-factor, an analog that normally blocks these responses. The M54I mutation appeared to affect only ligand specificity. The other mutations conferred additional effects on signaling or recovery. Two mutants were more sensitive to alpha-factor than wild type (S145L, A229V). One mutant was more sensitive to alpha-factor-induced cell cycle arrest initially, but then recovered more efficiently (S145L-S219L). One mutant (L255S-S288P) conferred positive pheromone responses to alpha-factor as assayed by FUS1-lacZ reporter induction, but did not display growth arrest. The hydrophobic receptor core thus appears to control activation by some ligands and to play roles in aspects of signal transduction and recovery.
机译：酿酒酵母α因子受体中的突变导致了对克鲁维酵母酵母α因子响应的改善，已被鉴定并测序。通过体内筛选从携带随机诱变的受体基因（STE2）质粒的细胞中分离突变体。五个突变导致受体核心的疏水性片段取代（M54I，S145L，S145L-S219L，A229V，L255S-S288P）。值得注意的是，表达这些突变受体的菌株对desTrp1，Ala3-α-因子（一种通常阻断这些应答的类似物）表现出正信息素应答。 M54I突变似乎只影响配体特异性。其他突变赋予信号传导或恢复额外的作用。两个突变体比野生型（S145L，A229V）对α因子更敏感。一个突变体最初对α-因子诱导的细胞周期停滞更加敏感，但随后更有效地恢复（S145L-S219L）。如FUS1-lacZ报告基因诱导检测的，一个突变体（L255S-S288P）赋予了对α因子的正信息素反应，但未显示出生长停滞。因此，疏水性受体核心似乎可以控制某些配体的活化，并在信号转导和恢复方面发挥作用。

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