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首页> 外文期刊>Molecular and Cellular Biology >NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes.
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NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes.

机译：酿酒酵母中线粒体o色突变的NAM9核抑制子编码一种与来自叶绿体，细菌和真核生物的S4核糖体蛋白同源的蛋白。

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We report the genetic characterization, molecular cloning, and sequencing of a novel nuclear suppressor, the NAM9 gene from Saccharomyces cerevisiae, which acts on mutations of mitochondrial DNA. The strain NAM9-1 was isolated as a respiration-competent revertant of a mitochondrial mit mutant which carries the V25 ochre mutation in the oxi1 gene. Genetic characterization of the NAM9-1 mutation has shown that it is a nuclear dominant omnipotent suppressor alleviating several mutations in all four mitochondrial genes tested and has suggested its informational, and probably ribosomal, character. The NAM9 gene was cloned by transformation of the recipient oxi1-V25 mutant to respiration competence by using a gene bank from the NAM9-1 rho o strain. Orthogonal-field alternation gel electrophoresis analysis and genetic mapping localized the NAM9 gene on the right arm of chromosome XIV. Sequence analysis of the NAM9 gene showed that it encodes a basic protein of 485 amino acids with a presequence that could target the protein to the mitochondrial matrix. The N-terminal sequence of 200 amino acids of the deduced NAM9 product strongly resembles the S4 ribosomal proteins from chloroplasts and bacteria. Significant although less extensive similarity was found with ribosomal cytoplasmic proteins from lower eucaryotes, including S. cerevisiae. Chromosomal inactivation of the NAM9+ gene is not lethal to the cell but leads to respiration deficiency and loss of mitochondrial DNA integrity. We conclude that the NAM9 gene product is a mitochondrial ribosomal counterpart of S4 ribosomal proteins found in other systems and that the suppressor acts through decreasing the fidelity of translation.
机译：我们报告了遗传特征，分子克隆和测序的一种新型的核抑制剂，从酿酒酵母，其作用于线粒体DNA突变的NAM9基因。 NAM9-1菌株是作为线粒体mit突变体的具有呼吸功能的回复株而分离的，该突变体在oxi1基因中携带V25茶黄突变。 NAM9-1突变的遗传学特征表明，它是一种核显性全能抑制剂，可缓解所有四个测试的线粒体基因中的几个突变，并暗示了其信息性和核糖体性状。使用来自NAM9-1 rho o菌株的基因库，通过将受体oxi1-V25突变体转化为呼吸能力来克隆NAM9基因。正交场交替凝胶电泳分析和遗传定位将NAM9基因定位在XIV染色体的右臂上。 NAM9基因的序列分析表明，该基因编码一个485个氨基酸的基本蛋白质，并且具有可以将该蛋白质靶向线粒体基质的序列。推导的NAM9产物的200个氨基酸的N末端序列非常类似于来自叶绿体和细菌的S4核糖体蛋白。尽管与低等真核生物（包括酿酒酵母）的核糖体胞质蛋白的相似性较差，但意义不大。 NAM9 +基因的染色体失活对细胞没有致死性，但会导致呼吸不足和线粒体DNA完整性丧失。我们得出的结论是，NAM9基因产物是在其他系统中发现的S4核糖体蛋白的线粒体核糖体对应物，并且抑制子通过降低翻译的保真度起作用。

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