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首页> 外文期刊>Molecular and Cellular Biology >An essential yeast gene with homology to the exonuclease-encoding XRN1/KEM1 gene also encodes a protein with exoribonuclease activity.
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An essential yeast gene with homology to the exonuclease-encoding XRN1/KEM1 gene also encodes a protein with exoribonuclease activity.

机译：与编码核酸外切酶的XRN1 / KEM1基因具有同源性的基本酵母基因还编码具有核酸外切酶活性的蛋白质。

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摘要

An essential gene, designated HKE1/RAT1, has been isolated from the yeast Saccharomyces cerevisiae and characterized. The gene encodes a protein of 116 kDa (p116) and has significant homology to another yeast gene (XRN1/KEM1) encoding a related protein (p175) with 5'-->3' exonuclease activity as well as activities involving chromosomal DNA pairing and mechanics. Preliminary analysis of an hke1ts mutant reveals a precipitous decline in the translation of mRNA at the nonpermissive temperature. Sporulation of heterozygous HKE1/hke1::URA3 diploids reveals that this gene, unlike the highly related XRN1/KEM1 gene, is essential for cell viability. Overexpression of the homologous gene product, p175, failed to rescue cells lacking a functional p116. In vitro studies demonstrate that p116 is a protein with 5'-->3' exoribonuclease activity, a major activity of the related p175. An immunoreactive RNase activity of 116 kDa is abolished with antiserum against p116. Both the level of this protein and the RNase activity correlate with HKE1 gene dosage. The RNase activity purifies coincidentally with a previously described 116-kDa RNase having 5'-->3' exoribonuclease activity.
机译：从啤酒酵母中分离出了必需基因，命名为HKE1 / RAT1。该基因编码一个116 kDa的蛋白质（p116），与另一个酵母基因（XRN1 / KEM1）具有明显的同源性，该基因编码一个具有5'-> 3'核酸外切酶活性以及涉及染色体DNA配对和机械师。对hke1ts突变体的初步分析显示，在非允许温度下，mRNA的翻译急剧下降。杂合的HKE1 / hke1 :: URA3二倍体的孢子形成表明，与高度相关的XRN1 / KEM1基因不同，该基因对细胞活力至关重要。同源基因产物p175的过表达不能拯救缺乏功能性p116的细胞。体外研究表明，p116是一种具有5'-> 3'核糖核酸外切酶活性的蛋白，是相关p175的主要活性。用针对p116的抗血清消除了116 kDa的免疫反应性RNase活性。该蛋白质的水平和RNA酶活性均与HKE1基因剂量相关。 RNase活性与先前描述的具有5'-> 3'核糖核酸外切酶活性的116-kDa RNase一致地纯化。

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