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首页> 外文期刊>Molecular and Cellular Biology >Identical components of yeast transcription factor IIIB are required and sufficient for transcription of TATA box-containing and TATA-less genes.
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Identical components of yeast transcription factor IIIB are required and sufficient for transcription of TATA box-containing and TATA-less genes.

机译：酵母转录因子IIIB的相同成分对于包含TATA盒和不含TATA的基因的转录是必需的且足够。

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摘要

Specific transcription by RNA polymerase III requires recognition of the promoter-bound transcription factor IIIB (TFIIIB), of which the TATA-binding protein (TBP) is a subunit. The recruitment of TFIIIB to TATA-less genes is mediated by protein-protein interactions with transcription factor IIIC (TFIIIC) bound to the box A and box B elements. Here we examine interactions involved in the recruitment of TFIIIB to the TATA element-containing yeast U6 small nuclear RNA gene SNR6. TFIIIC is not required for the formation of TFIIIB-SNR6 gene complexes with purified components. The same three components of TFIIIB that are necessary for TFIIIC-dependent transcription of tRNA genes (recombinant TBP and Brf and the denaturing-gel-purified 90-kDa subunit) are required and sufficient for TATA box-directed U6 transcription. Despite its TFIIIC-independent, DNA sequence-dependent assembly, the TFIIIB-SNR6 complex shares important features with tDNA- and 5S rDNA-TFIIIB complexes, such as extent and location of footprint, stability, and resistance to heparin. These properties are clearly distinct from those of a TBP-SNR6 complex. In the SNR6 gene, box B, the primary binding site for TFIIIC, is suboptimally spaced relative to box A. At limiting TBP concentrations and on bare DNA, TFIIIC stimulates the formation of TFIIIB complexes with SNR6 but contributes poorly, at best, to the formation of properly placed complexes.
机译：RNA聚合酶III的特异性转录需要识别与启动子结合的转录因子IIIB（TFIIIB），其中TATA结合蛋白（TBP）是其中的一个亚基。 TFIIIB募集到无TATA的基因是通过与框A和框B元件结合的转录因子IIIC（TFIIIC）的蛋白质相互作用来介导的。在这里，我们检查了涉及TFIIIB募集到含TATA元素的酵母U6小核RNA基因SNR6的相互作用。 TFIIIC不需要与纯化成分形成TFIIIB-SNR6基因复合物。 TFIIIB依赖于tRNA基因的TFIIIC依赖性转录（重组TBP和Brf以及变性凝胶纯化的90-kDa亚基）必需的三个相同成分，对于TATA盒定向U6转录是足够的。尽管其TFIIIC独立，DNA序列依赖性装配，但TFIIIB-SNR6复合物与tDNA和5S rDNA-TFIIIB复合物具有重要的特征，例如足迹的范围和位置，稳定性和对肝素的抵抗力。这些性质与TBP-SNR6复合物的性质明显不同。在SNR6基因中，框B（TFIIIC的主要结合位点）相对于框A处于最佳间隔。在限制TBP浓度和裸露的DNA上，TFIIIC刺激与SNR6形成TFIIIB复合物，但最多不会对SNRIII做出贡献。形成适当放置的复合物。

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