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首页> 外文期刊>Molecular and Cellular Biology >Identification of a candidate c-mos repressor that restricts transcription of germ cell-specific genes.
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Identification of a candidate c-mos repressor that restricts transcription of germ cell-specific genes.

机译：鉴定限制生殖细胞特异性基因转录的候选c-mos阻遏物。

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The c-mos proto-oncogene is specifically expressed in female and male germ cells. Previous studies identified a negative regulatory element (NRE) upstream of the c-mos promoter that suppresses c-mos transcription in transfected NIH 3T3 cells. In this study, we used gel shift assays to detect proteins in nuclear extracts of NIH 3T3 cells that bind to the c-mos NRE in a sequence-specific manner. One protein was found to bind to a region of the NRE which was shown by site-directed mutagenesis to be required for suppression of c-mos transcription. This factor was present in nuclear extracts of several somatic cell lines and tissues but not in male germ cells in which c-mos is transcribed, suggesting that it is a somatic cell repressor of c-mos transcription. The binding site of the candidate repressor within the c-mos NRE consists of sequences related to putative NREs identified in two other male germ cell-specific genes (encoding protamine 2 and phosphoglycerate kinase 2). The c-mos repressor bound and could be UV cross-linked to these protamine 2 and phosphoglycerate kinase 2 gene sequences as a protein with an apparent molecular mass of approximately 30 kDa. The repressor binding site is also conserved in two other germ cell-specific genes (encoding testis-specific cytochrome c and heat shock-like protein 70), suggesting that the c-mos repressor may be generally involved in suppressing transcription of germ cell-specific genes in somatic cells.
机译：c-mos原癌基因在雌性和雄性生殖细胞中特异性表达。先前的研究确定了c-mos启动子上游的负调控元件（NRE），该元件可抑制转染的NIH 3T3细胞中的c-mos转录。在这项研究中，我们使用凝胶位移分析法检测了NIH 3T3细胞核提取物中以序列特异性方式与c-mos NRE结合的蛋白质。发现一种蛋白质结合到NRE的区域，这通过定点诱变显示抑制c-mos转录是必需的。此因子存在于几种体细胞系和组织的核提取物中，但不存在于转录有c-mos的雄性生殖细胞中，这表明它是c-mos转录的体细胞阻遏物。候选阻遏物在c-mos NRE中的结合位点由与在其他两个雄性生殖细胞特异性基因（编码鱼精蛋白2和磷酸甘油酸激酶2）中鉴定的推定NRE相关的序列组成。 c-mos阻遏物作为表观分子量约为30 kDa的蛋白质结合并可以与这些鱼精蛋白2和磷酸甘油酸激酶2基因序列进行紫外线交联。阻遏物结合位点在另外两个生殖细胞特异性基因（编码睾丸特异性细胞色素c和热休克样蛋白70）中也保守，这表明c-mos阻遏物通常可以参与抑制生殖细胞特异性转录体细胞中的基因。

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