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An alternative eukaryotic DNA excision repair pathway.

机译：替代的真核DNA切除修复途径。

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DNA lesions induced by UV light, cyclobutane pyrimidine dimers, and (6-4)pyrimidine pyrimidones are known to be repaired by the process of nucleotide excision repair (NER). However, in the fission yeast Schizosaccharomyces pombe, studies have demonstrated that at least two mechanisms for excising UV photo-products exist; NER and a second, previously unidentified process. Recently we reported that S. pombe contains a DNA endonuclease, SPDE, which recognizes and cleaves at a position immediately adjacent to cyclobutane pyrimidine dimers and (6-4)pyrimidine pyrimidones. Here we report that the UV-sensitive S. pombe rad12-502 mutant lacks SPDE activity. In addition, extracts prepared from the rad12-502 mutant are deficient in DNA excision repair, as demonstrated in an in vitro excision repair assay. DNA repair activity was restored to wild-type levels in extracts prepared from rad12-502 cells by the addition of partially purified SPDE to in vitro repair reaction mixtures. When the rad12-502 mutant was crossed with the NER rad13-A mutant, the resulting double mutant was much more sensitive to UV radiation than either single mutant, demonstrating that the rad12 gene product functions in a DNA repair pathway distinct from NER. These data directly link SPDE to this alternative excision repair process. We propose that the SPDE-dependent DNA repair pathway is the second DNA excision repair process present in S. pombe.
机译：已知由紫外线，环丁烷嘧啶二聚体和（6-4）嘧啶嘧啶酮诱导的DNA损伤可通过核苷酸切除修复（NER）的过程修复。然而，在裂变酵母粟酒裂殖酵母中，研究表明，存在至少两种去除紫外线光产物的机制。 NER和第二个以前未知的过程。最近，我们报道了粟酒裂殖酵母含有一种DNA核酸内切酶SPDE，它可以在紧邻环丁烷嘧啶二聚体和（6-4）嘧啶嘧啶酮的位置识别并切割。在这里我们报告紫外线敏感的粟酒裂殖酵母rad12-502突变体缺乏SPDE活性。此外，从rad12-502突变体制备的提取物缺乏DNA切除修复，如体外切除修复测定所示。通过向体外修复反应混合物中添加部分纯化的SPDE，可将rad12-502细胞制备的提取物中的DNA修复活性恢复至野生型水平。当rad12-502突变体与NER rad13-A突变体杂交时，所得的双突变体比任一单个突变体对紫外线辐射敏感得多，这表明rad12基因产物在不同于NER的DNA修复途径中起作用。这些数据直接将SPDE链接到此替代性切除修复过程。我们建议依赖SPDE的DNA修复途径是粟酒裂殖酵母中存在的第二个DNA切除修复过程。

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