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首页> 外文期刊>Molecular and Cellular Biology >Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae.
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Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae.

机译：酿酒酵母芽萌发基因BEM4的鉴定及其与rho型GTPases的相互作用。

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The Rho-type GTPase Cdc42p is required for cell polarization and bud emergence in Saccharomyces cerevisiae. To identify genes whose functions are linked to CDC42, we screened for (i) multicopy suppressors of a Ts- cdc42 mutant, (ii) mutants that require multiple copies of CDC42 for survival, and (iii) mutations that display synthetic lethality with a partial-loss-of-function allele of CDC24, which encodes a guanine nucleotide exchange factor for Cdc42p. In all three screens, we identified a new gene, BEM4. Cells from which BEM4 was deleted were inviable at 37 degrees C. These cells became unbudded, large, and round, consistent with a model in which Bem4p acts together with Cdc42p in polarity establishment and bud emergence. In some strains, the ability of CDC42 to serve as a multicopy suppressor of the Ts- growth defect of deltabem4 cells required co-overexpression of Rho1p, which is an essential Rho-type GTPase necessary for cell wall integrity. This finding suggests that Bem4p also affects Rho1p function. Bem4p displayed two-hybrid interactions with Cdc42p, Rho1p, and two of the three other known yeast Rho-type GTPases, suggesting that Bem4p can interact with multiple Rho-type GTPases. Models for the role of Bem4p include that it serves as a chaperone or modulates the interaction of these GTPases with one or more of their targets or regulators.
机译：Rho型GTPase Cdc42p是酿酒酵母中细胞极化和芽萌发所必需的。为了鉴定功能与CDC42相关的基因，我们筛选了（i）Ts-cdc42突变体的多拷贝抑制剂，（ii）需要CDC42多个拷贝才能存活的突变体，以及（iii）具有部分合成杀伤力的突变功能缺失的CDC24等位基因，其编码Cdc42p的鸟嘌呤核苷酸交换因子。在所有三个屏幕中，我们鉴定了一个新基因BEM4。删除了BEM4的细胞在37摄氏度时是可行的。这些细胞变得没有预算，大而圆形，这与Bem4p和Cdc42p在极性建立和芽萌发中起作用的模型一致。在某些菌株中，CDC42用作deltabem4细胞Ts生长缺陷的多拷贝抑制剂的能力需要Rho1p的共过量表达，Rho1p是细胞壁完整性必不可少的必需Rho型GTPase。这一发现表明Bem4p也影响Rho1p功能。 Bem4p与Cdc42p，Rho1p和其他三个已知的酵母Rho型GTPases中的两个显示了两种杂交，表明Bem4p可以与多个Rho型GTPases相互作用。 Bem4p作用的模型包括它充当伴侣或调节这些GTPases与它们的一个或多个靶标或调节剂的相互作用。

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