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首页> 外文期刊>Molecular and Cellular Biology >Induction and maintenance of 2',5'-oligoadenylate synthetase in interferon-treated chicken embryo cells.
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Induction and maintenance of 2',5'-oligoadenylate synthetase in interferon-treated chicken embryo cells.

机译：干扰素处理的鸡胚细胞中2'，5'-寡腺苷酸合成酶的诱导和维持。

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Treatment of primary cultures of chicken embryo cells with homologous interferon results in a substantial increase in the level of 2',5'-oligoadenylate synthetase activity that can be detected in cell extracts. This increase can be prevented by inhibitors of RNA or protein synthesis and is thus thought to represent the induction of an interferon-inducible gene, perhaps the 2',5'-oligoadenylate synthetase gene itself. To examine this response in greater detail, we studied its kinetics under the following conditions: (i) cessation of interferon treatment after different lengths of time, (ii) delayed inhibition of RNA or protein synthesis, and (iii) combinations of these treatments. The results showed that in cells treated continuously with interferon, the enzyme level reached a peak after 9 h of treatment and then decreased with a half-life of about 30 h, despite the continued presence of interferon. Removal of interferon during induction reduced the peak level of activity that was attained and somewhat accelerated its decline but did not otherwise affect the time-course of the response. On the other hand, removal of interferon after maximum induction clearly accelerated the decay of enzyme activity. This process could be delayed by inhibitors of protein synthesis, which effectively stabilized the induced enzyme. This behavior is reminiscent of other inducible enzymes, such as the steroid-induced tyrosine aminotransferase, and suggests that the level of 2',5'-oligoadenylate synthetase, which is also inducible by steroid hormones in some cell types, is subject to similar control mechanisms.
机译：用同源干扰素处理鸡胚细胞的原代培养物会导致可在细胞提取物中检测到的2'，5'-寡腺苷酸合成酶活性水平大大提高。这种增加可以通过RNA或蛋白质合成的抑制剂来阻止，因此被认为代表了干扰素诱导基因的诱导，也许是2'，5'-寡腺苷酸合成酶基因本身的诱导。为了更详细地检查这种反应，我们在以下条件下研究了其动力学：（i）在不同时间长度后停止干扰素治疗，（ii）延迟抑制RNA或蛋白质合成，以及（iii）这些治疗的组合。结果表明，在连续用干扰素处理的细胞中，尽管干扰素持续存在，但酶水平在处理9小时后达到峰值，然后以约30小时的半衰期降低。在诱导过程中去除干扰素可降低所达到的活性峰值水平，并在一定程度上加速其下降，但不会影响反应的时间过程。另一方面，在最大诱导后去除干扰素显然加速了酶活性的衰减。蛋白质合成抑制剂可有效地稳定诱导的酶，从而延迟该过程。此行为使人联想到其他诱导酶，例如类固醇诱导的酪氨酸氨基转移酶，并表明2'，5'-寡腺苷酸合成酶的水平（在某些细胞类型中也可由类固醇激素诱导）也受到类似的控制机制。

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