Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers.

P S Linsley; L Siminovitch

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Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers.

机译：使用克隆的选择性标记比较表型表达和基因型转化。

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The frequency of phenotypic expression of the herpes simplex virus type 1 tk and Escherichia coli gpt genes was compared with the frequency of genotypic transformation after calcium phosphate-mediated DNA transfection of a number of tk- and hprt- cell lines. In three of the five lines tested, the frequency of phenotypic expression was at most 10-fold higher than that of genotypic transformation as indicated by frequency of HAT resistance. The remaining two lines showed phenotypic responses which were 50- to 100-fold greater than the genotypic responses. The data indicate that the efficiency of DNA-mediated transformation with some cell lines can be limited by events after the uptake and expression of transfected DNA.

机译：将单纯性疱疹病毒1型tk和大肠杆菌gpt基因的表型表达频率与磷酸钙介导的许多tk和hprt细胞系的DNA转染后的基因型转化频率进行了比较。在五个测试的品系中的三个品系中，表型表达的频率最多比基因型转化的频率高10倍，如HAT耐性的频率所示。其余两行表现出的表型应答比基因型应答高50至100倍。数据表明，DNA介导的某些细胞系转化的效率可能受到转染DNA摄取和表达后事件的限制。

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《Molecular and Cellular Biology》 |1982年第5期|共5页
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P S Linsley; L Siminovitch;
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中图分类细胞生物学;
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6. Comparison of phenotypic expression with genotypic transformation by using cloned selectable markers. [O] . P S Linsley, L Siminovitch 1982

机译：使用克隆的选择性标记比较表型表达和基因型转化。
7. Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers. [O] . Linsley, P S, Siminovitch, L 1982

机译：使用克隆的选择性标记比较表型表达和基因型转化。

Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers.

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